论文部分内容阅读
采用ｆｕｒａ－２双波长荧光法，测定了孵化第６至１６天鸡胚脑细胞内游离Ｃａ２＋的浓度。并且利用回转器模拟微重力的生物效应，研究了不同胚龄鸡胚旋转不同时间后，脑细胞内游离Ｃａ２＋水平的变化。结果表明，孵化第８至１６天鸡胚旋转不同时间后，其脑细胞内游离Ｃａ２＋浓度均有所下降，其中第１０天鸡胚旋转４或７小时及第１３天鸡胚旋转２４小时后，脑细胞内Ｃａ２＋浓度比对照组显著降低（ｐ＜０．０１）。第１０天和１３天鸡胚分别旋转４和２４小时后再静置恢复相同时间，细胞内Ｃａ２＋浓度均有所增加，但仍低于对照组水平。结果显示，回转器旋转引起的鸡胚脑细胞内游离Ｃａ２＋水平降低是可逆的
Fura-2 dual-wavelength fluorescence method was used to determine the concentration of free Ca2 + in chick embryo brain cells on the 6th to 16th day after hatching. The biological effects of microgravity were simulated by gyroscope to study the changes of intracellular free Ca2 + levels in different embryonic chicken embryos at different time points. The results showed that the intracellular free Ca2 + concentration decreased after the chicken embryos were spun from the 8th to the 16th day after hatching for different time. After the chicken embryos were rotated for 4 or 7 hours on the 10th day and the chicken embryos were rotated for 24 hours on the 13th day, Ca2 + concentration in brain cells was significantly lower than that in control group (p <0.01). On the 10th and 13th day, the chicken embryos were respectively rotated for 4 and 24 hours and then resumed for the same time. The intracellular Ca2 + concentration increased but remained lower than that of the control group. The results showed that the reduction of intracellular free Ca2 + level in chicken embryo brain cells caused by gyrator rotation was reversible