论文部分内容阅读
目的研究南宁市某医院门诊急性胃肠炎病例中GⅠ型诺如病毒的感染状况。方法应用荧光定量RT-PCR法对2007年1月～2008年12月南宁市某医院门诊腹泻病例标本进行GⅠ型诺如病毒RNA检测,并对GⅠ型阳性标本进行测序验证。结果在所检测的696份粪便悬液标本中,检出4份GⅠ型阳性,经核酸测序证实为GⅠ型诺如病毒。结论在广西的急性胃肠炎病例中存在GⅠ型诺如病毒感染。
Objective To study the infection status of G Ⅰ norovirus in the outpatient acute gastroenteritis in a hospital in Nanning. Methods The G Ⅰ norovirus RNA of outpatients with diarrhea in a hospital of Nanning City from January 2007 to December 2008 was detected by real-time fluorescence quantitative RT-PCR, and the GⅠ positive specimens were verified by sequencing. RESULTS: Of the 696 fecal samples tested, 4 were positive for type GⅠ, and confirmed to be type Ⅰ norovirus by nucleic acid sequencing. Conclusion G Ⅰ norovirus infection exists in Guangxi cases of acute gastroenteritis.