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目前常用疫苗攻击试验(如小鼠效力试验)对人用和兽用狂犬病灭活疫苗进行效力检定,本文讨论了体外ELISA法代替体内效力试验的可行性.人用冻干狂犬病灭活疫苗选用原代狗肾细胞培养的PM株(PM-DKC)制备,兽用液体灭活疫苗分别用PV、SAD、PM和Flury-LEP株制备.采用两种免疫捕获ELISA(GPELISA 6-15和GP ELISA 78-9)及一种竞争ELISA(GP ELISA)检测疫苗糖蛋白(GP)抗原,这些试验均以生物素标记的GP-单克隆抗体(McAb)2-22为指示抗体;采用竞争
At present, the commonly used vaccine attack test (such as mouse efficacy test) is used to test the effectiveness of inactivated vaccine for human and veterinary rabies in this paper, and the feasibility of replacing the in vivo efficacy test with in vitro ELISA is discussed. PM strains (PM-DKC) were cultured in dog kidney cells and the veterinary liquid inactivated vaccine was prepared using PV, SAD, PM and Flury-LEP strains, respectively.Two immunocapture ELISA (GP ELISA 6-15 and GP ELISA 78 -9) and a competitive ELISA (GP ELISA) for the detection of the vaccine glycoprotein (GP) antigen. These assays all use the biotinylated GP-monoclonal antibody (McAb) 2-22 as an indicator antibody;