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选用分布于水稻12条染色体上多态性好的44对SSR引物,对黑龙江省近年主栽品种和其他省份粳稻及国外粳稻品种共96份材料进行遗传多样性和群体结构分析,旨在了解黑龙江省水稻品种遗传结构和亲缘关系。结果表明,44对SSR引物共检测到141个等位基因,每个位点1~5个,平均3.2个;遗传相似系数(GS)变化范围为0.46~0.97,平均0.77;试验中第1、2、4、6号染色体SSR位点多态性高,而第12条染色体SSR位点多态性低;利用SSR标记分析数据和STRUCTURE 2.2软件分析,全部供试品种可划分为7个亚群,分别命名为POP1,POP2,POP3,POP4,POP5,POP6和POP7;STRUCTURE模型群体结构分析与Nei’s遗传距离构建邻位相连聚类图分析结果基本一致。
A total of 44 pairs of SSR primers with good polymorphism on 12 chromosomes of rice were used to analyze the genetic diversity and population structure of 96 cultivars in recent years in Heilongjiang Province, 96 japonica cultivars in other provinces and japonica rice varieties in other provinces. Genetic Structure and Genetic Relationship of Rice Varieties in Provinces. The results showed that a total of 141 alleles were detected by 44 pairs of SSR primers, with 1 ~ 5 loci per locus (average 3.2). The genetic similarity coefficient (GS) ranged from 0.46 to 0.97 with an average of 0.77. The SSR loci on chromosomes 2, 4 and 6 were highly polymorphic while the SSR locus on chromosome 12 was low. Based on SSR marker analysis data and STRUCTURE 2.2 software analysis, all the tested cultivars could be divided into seven subgroups , Which were named as POP1, POP2, POP3, POP4, POP5, POP6 and POP7, respectively. The STRUCTURE population structure analysis and Nei’s genetic distance construct the ortho-linked cluster analysis results are basically the same.