论文部分内容阅读
目的：建立恶性疟原虫谷氨酸富有蛋白（ＧＬＵＲＰ）基因分型诊断鉴别系统。方法：设计并合成两对针对恶性疟原虫ＧＬＵＲＰ基因的特异引物，采用套式聚合酶链反应（ＰＣＲ）技术，扩增ＧＬＵＲＰ基因Ｒ２多态区目的基因，并应用于泰国Ｂｏｒａｉ疟区恶性疟患者虫株基因分型。结果：在自然感染的恶性疟原虫株群中，ＧＬＵＲＰ基因存在明显的多态性。在１５４份恶性疟感染血样中，共检查出２９０个基因株，它们属于１２种ＤＮＡ片段长度不同的ＧＬＵＲＰ基因型；其中以７７０ｂｐ片段基因型较为常见，１１００ｂｐ片段基因型较少见。４３％以上病人为不同恶性疟原虫基因株混合感染者。在９个月的流行季节中，１２种不同基因株的频率构成比无明显变化。结论：建立ＧＬＵＲＰ基因分型诊断鉴别系统，将有助于疟原虫虫株分类和致病性研究，对疟疾的流行病学研究与防治具有实用意义。
Objective: To establish a differential diagnostic system for GLURP genotyping of Plasmodium falciparum. Methods: Two pairs of primers specific to the GLURP gene of Plasmodium falciparum were designed and synthesized. The target gene of GLURP gene R2 polymorphism was amplified by nested polymerase chain reaction (PCR) and applied to the patients with falciparum malaria in Borai malaria area of Thailand Insect strain genotyping. RESULTS: There was a significant polymorphism in the GLURP gene in naturally infected P. falciparum strains. Of the 154 blood samples collected from P. falciparum, 290 strains were found, which belonged to GLURP genotypes with 12 different DNA fragments. Among them, 770 bp genotypes were common, and 1100 bp genotypes were rare. More than 43% of patients with different Plasmodium falciparum strains mixed. In the 9-month epidemic season, there was no significant change in the frequency composition of 12 different genotypes. Conclusion: The establishment of GLURP genotyping diagnosis and identification system will contribute to the classification and pathogenicity of Plasmodium. It is of practical significance for the epidemiological study and prevention and treatment of malaria.