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免疫调节因子白细胞介素－２（ＩＬ－２）具有中枢镇痛作用，其功能位点由ＩＬ－２分子的第４４、４５和１０７、１１７位氨基酸残基共同组成。采用基因定点突变和ＰＣＲ技术，缺失ＩＬ－２分子第６６～９９位残基，改变第４４、４５位残基与１０７、１１７位残基的相对空间位置，发现其镇痛功能消失。结果提示了组成ＩＬ－２分子镇痛功能位点的氨基酸残基间相对空间位置排列的重要性。
Immunoregulatory factor interleukin-2 (IL-2) has central analgesic effect and its functional site consists of amino acid residues 44, 45 and 107, 117 of IL-2 molecule. Site-directed mutagenesis and PCR were used to delete the residues 66-99 of IL-2 and to change the relative spatial positions of residues 44 and 45 and residues 107 and 117. It was found that the analgesic function disappeared. The results suggest the importance of the relative spatial arrangement of amino acid residues that make up the functional site of IL-2 molecule analgesia.