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用~3H-TdR、~(51)Cr、~(125)I-UdR 分别标记YAC-1细胞,测定小鼠 NK 细胞活性 ,三种方法所获得的结果呈高度相关性,~3H-TdR 分别与~(51)Cr、~(125)I-UdR 相比以及~(51)Cr与~(125)I-UdR 相比的相关系数依次为 0.9917(P<0.001),0.9725(P<0.001),0.9478(P<0.005),表明三种释放试验均可用于检测细胞毒活性。用收集器收集残存的~(125)I-UdR 标记细胞,不仅其特异性释放率比常规测上清液的释放率要高10％左右,而且制样快速、简便。本文对三种方法的优缺点作了比较,认为~3H-TdR 释放试验作为检测细胞毒是目前国内切实可行的方法。
YAC-1 cells were labeled with ~ 3H-TdR, ~ (51) Cr, ~ (125) I-UdR and the activity of NK cells in mice was measured. The results obtained by the three methods were highly correlated The correlation coefficients of ~ (51) Cr and ~ (125) I-UdR were ~ 0.9917 (P <0.001) and 0.9725 (P <0.001) , 0.9478 (P <0.005), indicating that all three release assays are available for the detection of cytotoxic activity. Collecting the remaining 125 I-UdR labeled cells with a collector not only has a specific release rate of about 10% higher than that of the conventional supernatant, but also makes the preparation quick and easy. In this paper, the advantages and disadvantages of the three methods were compared. It is concluded that ~ 3H-TdR release assay is a feasible and feasible method for the detection of cytotoxicity in China.