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目的 研究亚硒酸钠对大鼠肝细胞增殖周期和DNA合成的影响。方法 选用雄性SD大鼠 ,每组 5只 ,用 5 ,10和 2 0 μmol/kg的亚硒酸钠腹腔注射染毒。用流式细胞术研究大鼠肝细胞增殖周期和DNA相对含量(DNARC) ,用单细胞凝胶电泳检测DNA损伤。结果 10和 2 0 μmol/kg的亚硒酸钠均可使大鼠肝细胞G0 /G1期细胞显著减少 ,5 μmol/kg的亚硒酸钠虽可使G0 /G1期细胞减少 ,但无显著性差异。S期和G2 /M期细胞以及增殖指数变化不明显。 5 ,10和 2 0 μmol/kg的亚硒酸钠可引起大鼠肝细胞DNARC下降 ,DNA损伤率较对照组显著增高 ,而且DNARC和DNA损伤率之间存在负相关关系 ,随DNA损伤率的增高 ,DNARC下降 ,相关系数为 :- 0 9887(P <0 0 1)。结论 一定剂量的亚硒酸钠不仅改变了大鼠肝细胞的增殖周期 ,还引起DNA损伤 ,并影响DNA合成 ,使DNA相对含量显著下降。
Objective To study the effect of sodium selenite on the proliferation and DNA synthesis of rat hepatocytes. Methods Male Sprague-Dawley rats were randomly divided into five groups and treated with intraperitoneal injection of 5, 10 and 20 μmol / kg sodium selenite. Flow cytometry was used to study the proliferation of rat hepatocytes and the relative content of DNA (DNARC). DNA damage was detected by single cell gel electrophoresis. Results Both sodium selenite at 10 and 20 μmol / kg significantly reduced the number of G0 / G1 phase cells in rat hepatocytes. Although sodium selenite at 5 μmol / kg reduced the number of cells at G0 / G1 phase but no significant difference Sex differences. S phase and G2 / M phase cells and proliferation index did not change significantly. 5, 10 and 20 μmol / kg of sodium selenite can cause DNARC decreased rat liver DNA damage rate was significantly higher than the control group, and DNARC and DNA damage rate was negatively correlated, with the DNA damage rate Increased, DNARC decreased, the correlation coefficient: - 0 9887 (P <0 0 1). Conclusion A certain dose of sodium selenite not only changes the proliferation cycle of rat hepatocytes, but also causes DNA damage and affects the DNA synthesis, resulting in a significant decrease in the relative content of DNA.