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经实验我们成功地建立了在细胞水平上筛选烟草抗黑胫病突变体的筛选体系。该体系的主要内容为:γ-射线500—2000拉德诱变高度感病品种的花药后用50—80％的黑胫病菌粗毒素为选择压力,筛选出抗毒素花粉植株,用离体叶片法测定选出抗病植株,再从后代鉴定中选出抗病性能够稳定遗传的突变系。γ-射线及高浓度毒素处理均能得到抗病植株。选自感病品种的花粉植株中约有9—50％是真正抗病的。这些抗病植株中有一部分的抗病性能够稳定遗传。用该法已从感病优质品种小黄金1025及乔庄黑苗中选出6个突变系。并自N.C.628(抗)×小黄金1025(感)及N.C.628(抗)×庆胜2号(感)的F_1花粉植株中选出4个抗病系。所有的抗病系经3—4代后均表现出稳定抗性。其中一个突变体(R400)的抗性似由不完全显性多基因控制。
Through experiments, we have successfully established a screening system for screening tobacco anti-shank mutant at the cellular level. The main contents of the system are as follows: γ-ray 500-2000 Radian mutagenesis highly susceptible varieties of anther with 50-80% of the black pepper throat crude toxin selection pressure, antitoxin pollen selected plants, The resistant plants were selected and the mutant lines with stable disease resistance were selected from the progeny identification. γ-ray and high concentrations of toxins can get resistant plants. About 9-50% of pollen plants selected from susceptible varieties are truly disease resistant. Some of these disease-resistant plants have a stable inheritance of disease resistance. The method has been selected from the susceptible varieties of small gold 1025 and Qiaozhuang black seedling selected six mutant lines. Four resistant lines were selected from F1 pollen plants of N.C.628 (resistant) × small gold 1025 (flu) and N.C.628 (resistant) × Qingsheng 2 (flu). All resistance lines showed stable resistance after 3-4 generations. The resistance of one of the mutants (R400) appears to be controlled by an imperfectly dominant polygene.