胰岛素毫微球肺部给药对正常大鼠的降血糖作用

• 作 者: 沈赞聪 张强 魏树礼
• 机 构: 北京医科大学药剂研究室!北京100083,北京医科大学药剂研究室!北京100083,北京医科大学药剂研究室!北京100083
• 出 处: 北京医科大学学报
• 发表日期: 1999年05期
• 关键词: 降血糖作用 呼吸 血糖变化 生物利用度 给药途径

目的：研究胰岛素毫微球（ｉｎｓｕｌｉｎｌｏａｄｅｄ ｎａｎｏｐａｒｔｉｃｌｅｓ，ＩＮＳＮＰ） 经大鼠肺部给药后的降血糖作用。方法：建立大鼠肺部给药的动物模型，通过葡萄糖氧化酶法测定血糖质量浓度来评价ＩＮＳＮＰ 经肺部给药后对大鼠的降血糖作用。结果：大鼠肺部分别给与５、１０ 、２０ ｕ·ｋｇ－ １ 的ＩＮＳＮＰ，以血糖下降至给药前的７０ ％ 以下所持续的时间（ｄｕｒａｔｉｏｎ ｂｅｌｏｗ ７０ ％ ｌｅｖｅｌ，ＤＢＬ７０％）为考察指标，５ｕ·ｋｇ－ １ 的ＩＮＳＮＰ的ＤＢＬ７０％ 与其对照溶液相近，而１０、２０ ｕ·ｋｇ－１的ＩＮＳＮＰ的ＤＢＬ７０ ％ 均明显大于各自的对照溶液。ＩＮＳＮＰ肺部给药的相对药理生物利用度达到５９．２％ 。结论：与胰岛素溶液相比，ＩＮＳＮＰ经大鼠肺部给药后能显著延长其血糖下降的时间。

Objective: To study the hypoglycemic effect of insulinloaded nanoparticles (INSNP) after pulmonary administration in rats. Methods: Animal models of pulmonary administration in rats were established. Glucose oxidase method was used to determine the blood glucose concentration to evaluate the hypoglycemic effect of INSNP administered to rats after pulmonary administration. Results: The rats were given 5, 10, 20 u · kg-1 of INS-NP respectively. The duration of blood glucose dropped below 70% before administration was below 70% level (DBL 70%) According to the index, the DBL70% of INSN with 5u · kg-1 was similar to that of the control solution, while the DBL70% of INSN with 10,20 u · kg-1 was significantly greater than that of the control solution. The relative pharmacological bioavailability of INSNP pulmonary administration reached 59.2%. Conclusion: Compared with insulin solution, INSNP significantly prolonged the decline of blood glucose after pulmonary administration in rats.