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目的建立同时测定含菊花提取物保健食品中的绿原酸、3,5-O-双咖啡酰基奎宁酸、木犀草苷和4,5-O-双咖啡酰基奎宁酸含量的高效液相色谱测定方法。方法采用70%甲醇为溶剂,超声提取样品。选择Waters Atlantis C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇和0.1%磷酸水溶液梯度洗脱,流速1.0 ml/min,进样量10μl,柱温35℃。检测波长选择每个化合物的最大波长进行检测。结果绿原酸在0.079μg~1.27μg、木犀草苷在0.007μg~0.114μg、3,5-O-双咖啡酰基奎宁酸在0.014μg~0.232μg,4,5-O-双咖啡酰基奎宁酸在0.015μg~0.236μg的浓度范围内线性关系良好,相关系数均达到0.9999,平均回收率在90%~107%之间(RSD 0.63%~6.6%,n=3)。结论本方法快捷、简便、准确。可用于同时测定保健食品中的绿原酸、木犀草苷、3,5-O-双咖啡酰基奎宁酸和4,5-O-双咖啡酰基奎宁酸。
OBJECTIVE To establish a HPLC method for the simultaneous determination of chlorogenic acid, 3,5-O-bis-caffeoylquinic acid, luteolin and 4,5-O-biscaffeoylquinic acid in chrysanthemum extract health food Chromatographic methods. Methods 70% methanol was used as the solvent and the samples were extracted by sonication. The Waters Atlantis C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of a gradient of methanol and 0.1% phosphoric acid. The flow rate was 1.0 ml / min. The injection volume was 10 μl and the column temperature was 35 ℃. Detection wavelength Select the maximum wavelength of each compound for detection. As a result, the concentration of chlorogenic acid in the range of 0.079 μg to 1.27 μg, luteolin in the range of 0.007 μg to 0.114 μg, and the amount of 3,5-O-biscaffeoylquinic acid in the range of 0.014 μg to 0.232 μg, 4,5-O- The correlation coefficient was 0.9999 and the average recovery was between 90% and 107% (RSD 0.63% -6.6%, n = 3) in the concentration range of 0.015μg ~ 0.236μg. Conclusion The method is fast, simple and accurate. It can be used for the simultaneous determination of chlorogenic acid, luteolin, 3,5-O-dicofectionylquinic acid and 4,5-O-dicofectionylquinic acid in health foods.