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目的:研究克癃胶囊对双氧水诱导的PC-3细胞氧化应激反应的保护作用。方法:体外培养PC-3细胞,建立双氧水诱导PC-3细胞氧化应激的模型,给予克癃胶囊进行干预。分为4组:正常组、模型组(H_2O_2 100μmol/L)、阴性对照组(tBHQ50μmol/L+H_2O_2100μmol/L)、克癃胶囊组(克癃方100μg/m L+H_2O_2 100μmol/L)以DCFH-DA为荧光探针,利用流式细胞仪检测细胞内活性氧自由基(ROS)含量,利用化学显色法测定PC-3细胞内还原型谷胱甘肽(GSH)含量,评价细胞氧化应激水平。结果:ROS、GSH水平模型组与正常组比较,阳性对照组、克癃胶囊组与模型组比较,差异均有统计学意义(P<0.01);ROS水平阳性对照组与克癃胶囊组比较,差异有统计学意义(P<0.01),但两组GSH水平比较,差异无统计学意义(P>0.05)。结论:克癃胶囊对双氧水诱导的PC-3细胞氧化应激具有保护作用。
Objective: To study the protective effect of Kelud capsule on the oxidative stress induced by hydrogen peroxide in PC-3 cells. Methods: PC-3 cells were cultured in vitro, and a model of oxidative stress induced by H2O2 in PC-3 cells was established. Ketone capsules were administered for intervention. The rats were divided into 4 groups: normal group, model group (H_2O_2 100μmol / L), negative control group (tBHQ50μmol / L + H_2O_2100μmol / L) -DA as a fluorescent probe, the content of reactive oxygen species (ROS) in cells was detected by flow cytometry, and the content of reduced glutathione (GSH) in PC-3 cells was determined by chemical colorimetry. Shock level. Results: Compared with the normal group, the levels of ROS and GSH in the model group and the normal group were significantly different (P <0.01). Compared with the KO group, The difference was statistically significant (P <0.01), but the two groups of GSH levels, the difference was not statistically significant (P> 0.05). Conclusion: KT capsule has a protective effect on H2O2-induced oxidative stress in PC-3 cells.