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AIM:To evaluate the metabolic effects associated withadministration of rhGH and/or Gin in parenterally fed,short-bowel rats.METHODS:Forty SD rats subjected to 75 % intestinalresection and maintained with parenteral nutritionwere randomly divided into 4 groups as follows:-rhGH,-Gin;-rhGH,+Gin;+rhGH,-Gin;+rhGH,+Gin.Bodyweight and nitrogen balance were evaluated daily.After6 days of PN,rats were killed,various organs weredissected and weighted,the carcasses were used foranalysis of body composition.Serum GH and IGF-1 weredetermined by RIA method.RESULTS:Weight loss in rats with rhGH(17.4±12.8 g)and rhGH+GIn(23.8±3.5 g)was significantly less thanrats with PN alone(29.6±6.9 g)and rats with Gin-supplemented PN(31.85±12.8 g),P<0.05.Theaccumulated NB in rats with rhGH(1252.9±294.3 mgN/d)and rhGH+GIn(1261.7±85.5 mg N/d)wassignificantly greater than those with PN alone(704.8±379.0 mg N/d)and with Gin-supplemented PN(856.7±284.4 mg N/d),P<0.05.The absolute weightof gastrocnemius muscle in rats with rhGH(2683.9±341.6 rag)and rhGH+GIn(2579.1±359.5 mg)wasgreater than those with PN alone(2176.3±167.1 mg)and with GIn-supplemented PN(2141.9±353.6 mg).Although the absolute weight of remnant smallintestine itself was not significantly different in 4experimental groups,the weight/length of thesegments was greater in rats with rhGH and/or Gin(48.7±5.5,52.7±4.1 and 67.4±5.3 respectively)thanthose with PN alone(47.8±5.0),there were synergisticeffects between rhGH and Gin in improvement of theweight/length of remnant small intestine,P<0.05.Analyses of body carcass composition showed that ahigher percentage of carcass weight as protein and alower percentage of carcass weight as fat wereoccurred in rats with rhGH(20.8±4.0,6.0±2.6)andrhGH+GIn(21.3±2.4,4.4±1.5)than those with PNalone(16.4±2.4,9.2±3.7)and with Gin-supplementedPN(17.8±3.0,6.3±2.0),rhGH had significant effectson alteration of body composition,P<0.05.Serum GHand IGF-1 concentration in rats with rhGH(5.221±0.8and 425.1±19.2 ng/ml respectively)and rhGH+GIn(5.507±1.0 and 461.1±49.9 ng/ml respectively)weregreater than those with PN alone(3.327±1.7 and 325.8±29.6 ng/ml respectively)and with Gin-supplementedPN(3.433±0.1 and 347.7±55.7 ng/ml respectively),P<0.01. CONCLUSION:rhGH significantly improves theanabolism in parenterally fed.short bowel rats,anaboliceffect with Gin is less dramatic,there is no synergisticeffect between rhGH and GIn in improvement of wholebody anabolism.IGF-1 plays an important part ingrowth-promoting effects of rhGH.
AIM: To evaluate the metabolic effects associated withadministration of rhGH and / or Gin in parenterally fed, short-bowel rats. METHODS: Forty SD rats subjected to 75% intestinal sorting and maintained with parenteral nutritionwere divided into 4 groups as follows: -rhGH, -Gin; -rhGH, + Gin; + rhGH, -Gin; + rhGH, + Gin.Bodyweight and nitrogen balance were evaluated daily. After 6 days of PN, rats were killed, various organs weredissected and weighted, the carcasses were used foranalysis of body composition. Serum GH and IGF-1 were determined by RIA method .RESULTS: Weight loss in rats with rhGH (17.4 ± 12.8 g) and rhGH + GIn (23.8 ± 3.5 g) was significantly less thanrats with PN alone (29.6 ± 6.9 g ) and rats with Gin-supplemented PN (31.85 ± 12.8 g) P <0.05.Theaccumulated NB in rats with rhGH (1252.9 ± 294.3 mgN / d) and rhGH + GIn (1261.7 ± 85.5 mg N / d) with PN alone (704.8 ± 379.0 mg N / d) and with Gin-supplemented PN (856.7 ± 284.4 mg N / d), P <0.05.The absolute weight of gastrocnemius muscle in rats with rhGH (2683.9 ± 341.6 rag) and rhGH + GIn (2579.1 ± 359.5 mg) wasgreater than those with PN alone (2176.3 ± 167.1 mg) and with GIn-supplemented PN (2141.9 ± 353.6 mg) .Although the absolute weight of remnant smallintestine itself was not significantly different in 4experimental groups, the weight / length of thesegments was greater in rats with rhGH and / or Gin (48.7 ± 5.5, 52.7 ± 4.1 and 67.4 ± 5.3 respectively), thanthose with PN alone (47.8 ± 5.0), there were synergisticeffects between rhGH and Gin in improvement of the weight / length of remnant small intestine, P <0.05.Analyses of body carcass composition showed that ahigher percentage of carcass weight as protein and alower percentage of carcass weight as fat wereoccurred in rats with rhGH ( 20.8 ± 4.0, 6.0 ± 2.6) and rhGH + GIn (21.3 ± 2.4, 4.4 ± 1.5) than those with PNalone (16.4 ± 2.4,9.2 ± 3.7) and with Gin-supplemented PN (17.8 ± 3.0, 6.3 ± 2.0) significant effects on alteration of body composition, P <0.05.Serum GH and IGF-1 concentration in rats with rhGH (5.221 ± 0.8 and 425.1 ± 19.2 ng / mlrespectively) and rhGH + GIn (5.507 ± 1.0 and 461.1 ± 49.9 ng / ml respectively) were compared to those with PN alone (3.327 ± 1.7 and 325.8 ± 29.6 ng / ml respectively) and with Gin- supplemented PN (3.433 ± 0.1 and 347.7 ± 55.7 ng / ml respectively), P <0.01 CONCLUSION: rhGH significantly improves theanabolism in parenterally fed.short bowel rats, anaboliceffect with Gin is less less dramatic, there is no synergisticeffect between rhGH and GIn in improvement of wholebody anabolism. IGF-1 plays an important part ingrowth-promoting effects of rhGH.