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背景:在国内本实验室曾首次报道脊髓胶状质及外侧脊核核区出现强的αo免疫反应性,与一些参与感觉调控的神经肽在该区的分布类似,提示鸟核苷酸调节蛋白可能与一级传入信息的传递有关。目的:采用切断大鼠单侧背根后观察胶状质内αo免疫反应性的改变。设计:随机对照动物实验。单位:华中科技大学同济医学院神经生物学教研室。材料:实验于1995-12/1996-12在华中科技大学同济医学院神经生物学教研室完成。选择SD健康成年大鼠15只,随机分为3组:①正常组5只(未经任何处理)。②切断背根组10只(右侧)。③对照组(未切断背根的左侧作为对照)。方法:大鼠在100g/L水合氯醛(300mg/kg)腹腔注射麻醉下,切断右侧腰1～3脊神经背根,术后存活48～60h。用兔抗鸟核苷酸调节蛋白αo亚单位多克隆抗血清,采用免疫组织化学方法来观察大鼠脊髓内αo免疫反应性,对鸟核苷酸调节蛋白进行定位,观察其在切断脊神经背根后的改变情况。主要观察指标:①正常鼠和对照组鼠脊髓后角Ⅰ～Ⅲ层及外侧脊核区的αo免疫反应性。②切断背根组鼠脊髓后角Ⅰ～Ⅲ层及外侧脊核区的αo免疫反应性。结果:15只大鼠均进入结果分析。①正常鼠和对照组鼠脊髓后角浅层(Ⅰ～Ⅲ)均出现强的αo免疫反应性,第二层(胶状质)最为密集,外侧脊核区也出现αo免疫反应性纤维网。切断背根后胶状质内αo免疫反应性明显降低。②αo免疫反应性吸光度定量分析,对照组内侧区0.847±0.081,切断背根组内侧区0.633±0.073(t=5.71,P<0.001);对照组中间区0.823±0.089,切断背根组中间区0.6604±0.074(t=6.90,P<0.001);对照组外侧区0.915±0.090,切断背根组外侧区0.656±0.077(t=10.31,P<0.001);对照组平均值0.852±0.084,切断背根组平均值0.639±0.078(t=10.23,P<0.001)。结论:胶状质内与一级伤害性刺激传入有关的神经元末梢内鸟核苷酸调节蛋白部分来源于一级感觉神经元,提示鸟核苷酸调节蛋白可能参与调控一级感觉的传递。
BACKGROUND: In our country, it was first reported in our laboratory that there is strong αo immunoreactivity in the glial and lateral nucleus of the spinal cord, which is similar to the distribution of some neuropeptides involved in sensory regulation in this region, suggesting that the nucleotide sequence of the regulatory protein May be related to the delivery of primary incoming information. OBJECTIVE: To observe the change of αo immunoreactivity in colloid after cutting off unilateral dorsal root in rats. Design: Randomized controlled animal experiments. Unit: Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology. MATERIALS: Experiments were performed at Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology from December 1995 to December 1996. Fifteen SD healthy adult rats were randomly divided into three groups: ① normal group of 5 (without any treatment). ② cut off the dorsal root group 10 (right). ③ control group (not cut off the left dorsal root as a control). Methods: Rats were anesthetized by intraperitoneal injection of chloral hydrate (300mg / kg) (100g / L), and the dorsal root of 1 ~ 3 spinal nerve was excised. The survival time was 48 ~ 60h. Using polyclonal antiserum of rabbit anti-avon regulatory protein αo subunit, immunohistochemistry was used to observe the αo immunoreactivity in the spinal cord of rats and the localization of the nucleotide regulatory protein was observed. After the change. MAIN OUTCOME MEASURES: ①αo immunoreactivity in the Ⅰ ~ Ⅲ layer and the lateral nucleus of the spinal cord in the normal rat and control rats. ② Cut off the αo immunoreactivity in the dorsal horn Ⅰ ~ Ⅲ of the dorsal horn and the lateral spinal nucleus in the dorsal root ganglion. Results: All 15 rats entered the result analysis. ① There was strong αo immunoreactivity in the superficial layers of the posterior horn (Ⅰ ~ Ⅲ) of normal rats and control rats, the second layer (gelatinous) was the most dense, and the αo immunoreactive fibrous web appeared in the lateral spinal nucleus. After cutting off the dorsal root, the immunoreactivity of αo in colloid is obviously reduced. (2) Quantitative analysis of αo immunoreactivity showed that the medial area of the control group was 0.847 ± 0.081, the medial area of the DRG group was 0.633 ± 0.073 (t = 5.71, P <0.001), the control group was 0.823 ± 0.089, and the middle of the DRG group was 0.6604 ± 0.074 (t = 6.90, P <0.001). The lateral area of the control group was 0.915 ± 0.090, the lateral area of the dorsal root was cut off 0.656 ± 0.077 (t = 10.31, P <0.001), while the control group was 0.852 ± 0.084 Group mean 0.639 ± 0.078 (t = 10.23, P <0.001). CONCLUSIONS: Part of the neuregulin in neuronal endothelium, which is related to the first-order nociceptive stimuli, originated from first-level sensory neurons, suggesting that it might be involved in the regulation of primary sensory transmission .