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通过对栉孔扇贝Chlamys farreri×虾夷扇贝Patinopecten yessoensis正反单对杂交子一代幼虫进行ISSR(Inter Simple Sequence Repeats)标记,分析了双亲位点在杂交子一代中的传递分离方式。从20个ISSR引物中筛选出引物807和834进行扩增,正交家系和反交家系均统计了70条清晰稳定的扩增带。正交家系中双亲和F_1共有位点占总位点数的38.6%,仅由母本或父本传递给F_1的位点分别占总位点数的27.1%和21.4%。反交家系中双亲和F_1共有位点占总位点数的34.3%,仅由母本或父本传递给F_1的位点分别占总位点数的25.7%和22.9%,表明双亲的遗传物质皆传递给了F_1代,证实属间杂交成功。实验结果表明,两家系中F_1均偏向各自的母本。另外,在F_1中还出现了较高比例的非孟德尔分离位点和非亲位点。
An ISSR (Inter Simple Sequence Repeats) marker was used to detect Chlamys farreri × Patinopecten yessoensis positive and negative pairs of hybrid F1 generation larvae, and the transmission and separation of the parents in the hybrid generation were analyzed. Primers 807 and 834 were screened from 20 ISSR primers for amplification, and 70 clear and stable amplification bands were counted in both orthologs and anti-crossing families. The parents and F_1 shared 38.6% of the total number of loci in orthogonal families and 27.1% and 21.4% of the total number of loci transmitted to F_1 by only the maternal or paternal parents respectively. The parents and F_1 shared 27.3% of the total loci in the crossbred families, and the loci transmitted to F_1 by only the maternal or paternal parents accounted for 25.7% and 22.9% of the total loci respectively, indicating that both parents’ genetic material were transmitted F_1 generation gave evidence of successful crossbreeding. The experimental results show that the two families F_1 are biased toward their respective maternal. In addition, a higher proportion of non-Mendelian and non-parental isolates also appeared in F_1.