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目的及时了解北京市昌平区2009年流感样病例中甲型H1N1流感病毒感染情况。方法依据《甲型H1N1流感病毒RT-PCR检测技术标准操作规程》,应用一组寡核苷酸引物和双重标记的TaqMan(探针,对呼吸道鼻咽拭子标本中的甲型H1N1流感病毒核酸进行定性检测[实时荧光定量(RT-PCR)法检测甲型H1N1流感病毒]。结果2009年5月18日至11月29日共检测流感样病例标本3647份,检测出甲型H1N1流感病毒核酸阳性标本214份(阳性检测率为5.87%),7月27日检出首例甲型H1N1流感病毒核酸阳性标本,9月14-27日阳性检出数最多(112例)。发病年龄集中在5~岁、10~岁、15~岁和20~岁组,发病人群学生占76.62%,97.40%的病例具有发热症状,其次为咳嗽(42.86%)和咽痛(31.17%)等。结论2009年昌平区流感样病例中甲型H1N1流感病毒感染以学生为主,发病具有明显年龄、人群差异。开展甲型H1N1流感病毒核酸检测,有助于了解甲型H1N1流感病毒感染情况,为临床的诊治以及合理制定甲型H1N1流感病毒防控策略提供依据。
Objective To understand the influenza A (H1N1) virus infection in 2009 influenza-like cases in Changping District, Beijing. Methods According to the Standard Operating Procedures of RT-PCR for Detection of Influenza A H1N1 Influenza Virus, a set of oligonucleotide primers and double-labeled TaqMan probe were used to detect the influenza A (H1N1) virus in respiratory nasopharyngeal swab samples (H1N1 influenza virus was detected by real-time fluorescence quantitative RT-PCR) .Results A total of 3647 samples of influenza-like illness were detected from May 18, 2009 to November 29, 2009. Influenza A (H1N1) virus nucleic acid Positive samples 214 (positive detection rate of 5.87%), the first case of influenza A H1N1 influenza virus nucleic acid positive samples was detected on July 27, the highest number of positive detection in September 14-27 (112 cases) .The incidence of age focused on The patients in the age group of 5 ~ 10, the age of 15, the age of 20 ~ 20 and the age group of 20 ~ 7 accounted for 76.62%, 97.40% of the patients had fever, followed by cough (42.86%) and sore throat (31.17%). Influenza A (H1N1) virus infection in Changping District was mainly student-centered, with obvious age and population differences.The detection of influenza A (H1N1) virus nucleic acid was helpful to understand the infection of influenza A Diagnosis and treatment and reasonable formulation of influenza A H1N1 influenza virus prevention and control strategy mention in accordance with.