论文部分内容阅读
向日葵(Helianthus annuus L.)是较耐盐的作物之一,品种间的耐盐碱能力存在差异。评价向日葵品种对盐胁迫的耐受能力,筛选耐盐性较强的品种对开发盐碱地、利用基因工程方法克隆和转化耐盐基因,进而提高向日葵产量具有重要意义。选用牙签、美葵5号和美葵2929 3个向日葵品种,用砂培法和组培法评价向日葵品种对NaCl的耐性。结果表明,向日葵3个品种在两种培养方法条件下,幼苗的叶数、下胚轴长在NaCl浓度间均存在显著差异,并且各品种组培法的叶数多于砂培法,差异性也小于砂培法。向日葵在NaCl胁迫下叶数、下胚轴长在两种培养方法间的相关系数均达极显著差异。当以下胚轴长作为向日葵对NaCl耐性鉴定指标时,用砂培和组培两种方法所得到的结果是一致的。
Sunflower (Helianthus annuus L.) is one of the more salt-tolerant crops, there is a difference in salt-tolerant capacity between cultivars. To evaluate the tolerance of sunflower varieties to salt stress, it is of great importance to develop salt-tolerant cultivars with high salt tolerance for cloning and transformation of salt-tolerant genes by genetic engineering, thus improving the yield of sunflower. Toothpick, Sunflower 5 and Sunflower 2929 were used to evaluate the tolerance of sunflower cultivars to NaCl by sand culture and tissue culture. The results showed that there were significant differences in seedling leaf number and hypocotyl length in NaCl concentration between the three cultivars of sunflower under the two culture conditions, Also smaller than sand culture. The correlation coefficients between leaf number and hypocotyl length of sunflower under the NaCl stress were significantly different between the two methods. When the following hypocotyls were used as indicators of tolerance to NaCl in sunflower, the results obtained by both sand culture and tissue culture methods were consistent.