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在根尖细胞染色体计数和N-分带鉴定基础上,通过花粉母细胞(PMC)减数分裂中期Ⅰ(MI)的染色体配对分析对本室育成的5个普通小麦-簇毛麦异附加系进行细胞学稳定性分析。在被测的30个株系121单株中,2n=44,43和42的植株分别占77.7％,14.0％和3.3％。在进行染色体配对分析的15个二体异附加系植株中,有8株平均每个PMC的二价体数超过21.6个,单价体数低于0.5个,没有出现或仅在个别细胞中出现三价体或四价体,在细胞学上已基本稳定。另有半数植株的二价体数虽然也超过21个,但全部是22个二价体的PMC频率相对较低,含有单价体、三价体和四价体的PMC频率相对较高,它们在细胞学上尚不稳定。为保特异附加系的稳定,必须经常进行细胞学稳定性鉴定。
Based on the chromosome counting and N-banding identification of root tip cells, five common wheat-cluster-wool alien addition lines bred in this laboratory were analyzed by chromosome pairing analysis of metaphase Ⅰ (MI) of pollen mother cells (PMC) Cytological stability analysis. Among 121 tested plants, 2n = 44, 43 and 42 plants accounted for 77.7%, 14.0% and 3.3%, respectively. Among the 15 isogenic lines of 15 isozymes, 8 of them had an average of more than 21.6 bivalents per PMC, less than 0.5 monovalent motifs, none or only three The valence or tetravalent body, in cytology has been basically stable. While the other half of the plants had more than 21 divalent numbers, the frequency of all PMCs with 22 bivalents was relatively low. The frequency of PMCs containing monovalent, trivalent and tetravalent was relatively high, Cytologically unstable. In order to ensure the stability of specific additional lines, cytological stability must be identified frequently.