论文部分内容阅读
目的对化学发光法检测的梅毒抗体S/CO低值样本用不同方法进行复检,探讨最佳的临床检测方案。方法于2013年1月1日-2013年5月20日对本科30 241例血清标本进行化学发光微粒子免疫(chemiluminesent micropaticle immunoassay,CMIA)筛查,S/CO为1.2～8.0的样本分别应用同一样本相同方法、同一样本不同方法(ELISA,TPPA)进行复检,并对TPPA复检结果为阴性的样本进行免疫印迹检测。结果 63例S/CO为1.2～8.0的样本中,同一样本相同方法的复检结果符合率为98.41%(62/63)。同一份样本不同方法的复检结果如下:ELISA法和TPPA法复检结果显示S/CO为1.2～3组的符合率为52.17%(12/23)和56.52%(13/23),S/CO为3～8的符合率为97.50%(39/40)和97.50%(39/40),两组的符合率差异均有统计学意义(P=0.000)。11例TPPA法复检结果为阴性样本经免疫印迹法再次复检,结果有9例为阴性,2例可疑,进一步检测该2例标本,其结果可疑为阴性。结论对于CMIA检测梅毒抗体S/CO低值样本,尤其是S/CO为1.2～3的样本,复检有助提高检测结果的准确性,可避免临床医疗纠纷。
Objective To detect the low value of S / CO of syphilis antibody detected by chemiluminescence method by different methods to explore the best clinical testing program. Methods From January 1, 2013 to May 20, 2013, 30 241 serum samples from undergraduate were screened by chemiluminesent micropaticle immunoassay (CMIA). Samples with S / CO of 1.2-8.0 were applied to the same sample The same method, the same sample different methods (ELISA, TPPA) for re-examination, and TPPA retest results were negative for Western blot detection. Results Among the 63 samples with S / CO of 1.2-8.0, the coincidence rate of the same sample with the same sample was 98.41% (62/63). The retest results of different methods of the same sample were as follows: The retest results of ELISA and TPPA showed that the coincidence rates of S / CO from 1.2 to 3 were 52.17% (12/23) and 56.52% (13/23), S / The coincidence rates of CO between 3 and 8 were 97.50% (39/40) and 97.50% (39/40), respectively. The coincidence rates of two groups were statistically significant (P = 0.000). 11 cases of negative results of TPPA retest were re-test by Western blotting, the results of 9 cases were negative, 2 cases of suspicious, further testing of the two specimens, the results were negative. Conclusions For the CMIA to detect low S / CO value of syphilis antibody, especially samples with S / CO of 1.2-3, the retest can help to improve the accuracy of test results and avoid clinical medical disputes.