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白细胞介素1β(Interleukin-1β,IL-1β)是一种重要的促炎细胞因子,在中枢神经系统中发挥着广泛的生物学功能。大量研究表明,IL-1β的作用非常复杂,在不同的模型和条件下作用不同,包括神经损伤或者神经毒性作用。电压门控钾通道调节神经元电学性质,也参与多种中枢神经系统的病理学过程。虽然IL-1β和钾通道都在脑损伤和脑疾病过程中发挥重要作用,但目前还很少有它们之间关系的研究报导。文章作者以原代培养的大鼠皮层神经元为材料,使用全细胞膜片钳技术,研究了10ng/mL的IL-1β在不同处理时间下对皮层神经元电压依赖性钾电流的影响。根据电流的性质,可以将记录到的钾电流分为瞬时外向电流(IA)为主的IA样电流和延迟整流电流(IK)为主的IK样电流两部分,结果显示:IL-1β处理8h对二者没有作用;处理24h可使IA样和IK样电流的幅度降低20%左右。以上结果提示IL-1β对大鼠皮层神经元电压门控钾电流具有抑制作用,并且这种抑制可能具有时间依赖性。
Interleukin-1β (IL-1β) is an important pro-inflammatory cytokine that plays a wide range of biological functions in the central nervous system. Numerous studies have shown that IL-1β is very complex and has different effects under different models and conditions, including nerve damage or neurotoxicity. Voltage-gated potassium channels regulate the electrical properties of neurons and are also involved in the pathological processes of a variety of central nervous systems. Although both IL-1β and potassium channels play an important role in the process of brain injury and brain diseases, there are few reports on the relationship between them. The authors used primary cultured rat cortical neurons as a material and studied the effect of 10 ng / mL IL-1β on voltage-dependent potassium currents in cortical neurons at different treatment times using whole-cell patch clamp techniques. According to the nature of the current, the recorded potassium current can be divided into two types: instantaneous outward current (IA) -based IA-like current and delayed rectifier current (IK) -concentrated IK-like current. The results showed that IL- No effect on the two; 24h treatment can reduce the magnitude of IA-like and IK-like currents by about 20%. The above results suggest that IL-1β has an inhibitory effect on voltage-gated potassium currents in rat cortical neurons, and this inhibition may be time-dependent.