论文部分内容阅读
目的 研究海人酸（ＫＡ）诱发癫痫后颗粒细胞轴突和树突发芽是否会引起齿状回内突触可塑性变化。方法 电镜下对海马齿状回分子层内突触的密度和突触连接面的弯曲形式等参数进行定量研究。结果１，ＫＡ注射后３ｄ，大鼠ＤＧ分子层内的突触数目明显减少；而ＫＡ注射后７ｄ，突触数目恢复到与对照组相似的状态。２．与对照组及ＫＡ庄射后３ｄ组相比，ＫＡ注射后７ｄ大鼠ＤＧ分子层内的突触中，笑型突触所占突触总数的比例显著减少，愁型突触所占突触总数的比例显著增加。结论 １．ＫＡ注射后 ７ｄ时 ＤＧ内颗粒细胞苔状纤维及树突发芽是功能性的。２．推测 ＫＡ注射后 ７ｄ时 ＤＧ内愁型突触增加的原因与发芽的苔状纤维释放谷氨酸增多有关。
Objective To investigate whether synaptic plasticity of dentate gyrus caused by granular cell axons and dendritic sprouts after kainic acid (KA) -induced epilepsy. Methods Electron microscopy quantitatively studied the density of synapses and the curved forms of synaptic connections in the dentate gyrus of hippocampus. The results showed that the number of synapses in the DG layer of rats decreased obviously after KA injection, and the number of synapses returned to the state similar to the control group 7 days after KA injection. 2. Compared with the control group and the KA group, the percentage of synapse in the synapse of DG in the molecular layer of DG in rats was significantly decreased at 7 days after KA injection. The proportion of the synapses The proportion of the total increased significantly. Conclusion 1. At 7 days after KA injection, granule cell mossy fibers and dendritic sprouts in DG were functional. 2. It is speculated that the reason for the increase of DG intraocular synapses at 7 days after KA injection is related to the increase of glutamate released by germinated mossy fibers.