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白血病患者的骨髓细胞染色体分析,由于有丝分裂相数量不多,染色体分散不佳和形态模糊等原因,应用于临床诊断殊感困难。 1976年Yunis报道用氨甲喋呤(MTX)使细胞同步化进行淋巴细胞培养,得到大量早期分裂相。本文作者参照Yunis法并稍加改进,对2例急性白血病(AL)、4例慢性粒细胞白血病(CGL)的骨髓细胞和外周血(不加PHA)的培养物采用MTX处理,也取得了满意的效果。其具体步骤如下: 吸取新鲜骨髓液注入含15％小牛血清和
The chromosome analysis of bone marrow cells in patients with leukemia is difficult to apply to clinical diagnosis because of the small number of mitotic phases, poor chromosome dispersion, and ambiguous morphology. In 1976, Yunis reported that cells were synchronized with methotrexate (MTX) for lymphocyte culture, resulting in a large number of early dividing phases. The authors of this article referred to the Yunis method and improved slightly. MTX was also used to treat 2 cases of acute leukemia (AL), 4 cases of bone marrow cells of chronic myeloid leukemia (CGL), and cultures of peripheral blood (without PHA). Effect. The specific steps are as follows: Infuse fresh bone marrow fluid with 15% calf serum and