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目的探讨三氧化二砷(As2O3)对人卵巢腺癌细胞株A2780细胞体外增殖和细胞周期的影响。方法①体外培养A2780细胞株,采用四唑盐比色法(MTT)测定不同浓度的As2O3对A2780细胞系胞增殖的影响,并绘制剂量-效应曲线。②采用流式细胞仪(FCM)测定细胞周期。结果As2O3有效抑制A2780细胞生长,呈时间及浓度依赖性(P<0.05);As2O3可将A2780细胞周期阻滞于G2/M,并呈浓度依赖性(P<0.05)。结论As2O3抑制体外培养的A2780细胞株生长,并将其阻滞在G2/M期,抑制肿瘤细胞增殖。
Objective To investigate the effects of arsenic trioxide (As2O3) on the proliferation and cell cycle of human ovarian cancer cell line A2780 in vitro. Methods ① A2780 cell line was cultured in vitro. The effects of different concentrations of As2O3 on the proliferation of A2780 cell line were determined by tetrazolium salt assay (MTT), and the dose-response curve was drawn. ② The cell cycle was determined by flow cytometry (FCM). Results As2O3 effectively inhibited the growth of A2780 cells in a time and concentration-dependent manner (P <0.05). As2O3 blocked the cell cycle of A2780 at G2 / M in a concentration-dependent manner (P <0.05). Conclusion As2O3 can inhibit the growth of A2780 cell line in vitro and block it in G2 / M phase and inhibit the proliferation of tumor cells.