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目的:为开展芦荟多糖的抗肿瘤作用机制提供理想药物。方法:采用水提醇沉法提取芦荟粗多糖;无水乙醇、丙酮、乙醚洗涤,三氯乙酸除蛋白,得精制芦荟粗多糖AP1;超滤膜截留,得不同分子量芦荟多糖AP11、AP12、AP13;蒽酮-浓硫酸法测定各多糖含量;考马斯亮蓝染色法测定各多糖中蛋白含量。结果:从库拉索芦荟凝胶冻干粉中提取的芦荟多糖AP1、AP11、AP12、AP13中多糖所占比例分别为58%、79%、84%和85%,蛋白含量低。结论:该提取工艺简单易行,可为开展芦荟多糖的抗肿瘤作用研究提供理想的实验药物。
Objective: To provide the ideal medicine for the anti-tumor mechanism of aloe polysaccharide. Methods: The aloe crude polysaccharides were extracted by water extraction and alcohol precipitation method; washed with anhydrous ethanol, acetone and ether and deproteinized with trichloroacetic acid to obtain aloe crude polysaccharide AP1; the ultrafiltration membrane was cut off to obtain aloe polysaccharides AP11, AP12 and AP13 with different molecular weights Anthrone-concentrated sulfuric acid method was used to determine the contents of polysaccharides. Coomassie brilliant blue staining was used to determine the content of polysaccharides. Results: The polysaccharides of AP1, AP11, AP12 and AP13 extracted from Aloe vera gel freeze-dried powder were 58%, 79%, 84% and 85%, respectively, with low protein content. Conclusion: The extraction process is simple and easy, which can provide an ideal experimental drug for the study of anti-tumor effect of Aloe polysaccharides.