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本实验通过静脉注入大剂量人 HDL,人 LDL 到正常和高 Ch 血症家兔体内;大剂量人 HDL,人 AcLDL 到正常家兔体内;探讨 HDL 对 LDL 及 AcLDL 渗入家兔主动脉壁的影响。灌注大剂量~(131)I-LDL 后,各组实验兔较对照免血浆放射活性均增加,半衰期延长,高 Ch 血症尤为明显。对照兔主动脉壁标记脂蛋白渗入率:高 Ch 血症组>AcLDL 组>LDL 组。HDL 对~(131)I-LDL 及~(131)I-AcLDL 在家兔主动脉壁渗入率的影响:LDL 组减少18.660%,高 Ch 血症组减少48.280%,AcLDL 组减少63.320%。主动脉壁组织自显影显示:HDL 减少~(131)I-LDL 及~(131)I-AcLDL 渗入主动脉壁。标记脂蛋白在主动脉弓、胸腹段的渗入率,实验兔均低于对照兔。以上结果揭示主动脉内皮细胞表面有类似AcLDL 受体的存在,当 HDL 浓度异常高时可以阻止 LDL 及 AcLDL 渗入完整主动脉内皮层进入内膜。
In this experiment, we injected large doses of human HDL intravenously, human LDL to normal and hyperchemia in rabbits; large doses of human HDL, human AcLDL to normal rabbits; explore the impact of HDL on LDL and AcLDL infiltration in rabbit aortic wall . After high doses of 131I-LDL were instilled, the plasma radioactivities of the rabbits in each group were higher than those in the controls, and the half-life was prolonged. Hyperchromicemia was particularly evident. Controlled rabbit aortic wall labeling lipoprotein infiltration rate: Hyper Chemia group> AcLDL group> LDL group. The effect of HDL on the infiltration rate of ~(131)I-LDL and ~(131)I-AcLDL in aortic wall of rabbits: a decrease of 18.660% in the LDL group, a 48.280% decrease in the hyperchromatemia group, and a 63.320% decrease in the AcLDL group. Autoradiography of the aortic wall revealed that HDL decreased ~131 I-LDL and ~131 I-AcLDL infiltrated the aortic wall. The infiltration rate of labeled lipoprotein in the aortic arch and thoracoabdominal segment was lower in experimental rabbits than in control rabbits. The above results revealed that the surface of aortic endothelial cells resembles the presence of AcLDL receptors. When the HDL concentration is abnormally high, LDL and AcLDL can be prevented from infiltrating the intact aortic endothelial layer into the intima.