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本研究以‘濮麦9号’和‘宁麦14号’为试验材料,探究低氮胁迫对小麦(Triticum aestivum)苗期中下部叶片膜脂组成的影响。结果表明低氮处理后‘濮麦9号’生物量下降幅度明显低于‘宁麦14号’,胁迫后‘濮麦9号’的叶绿素含量、光系统Ⅱ(PSII)最大光化学效率和光合速率都显著高于‘宁麦14号’。低氮胁迫下,两个小麦品种叶片中的单半乳糖甘油二酯(monogalactosyldiacylglycerol,MGDG)、双半乳糖甘油二酯(digalactosyldiacylglycerol,DGDG)及总脂含量都显著降低,但‘濮麦9号’叶片中的总脂、DGDG含量、DGDG/MGDG比值以及MGDG、磷脂(phospholipid,PL)和总脂的不饱和脂肪酸双键指数(DBI)都显著高于‘宁麦14号’。由此可见,低氮胁迫后小麦叶片膜脂组分及含量的不同变化可能是这两个小麦品种对低氮胁迫响应不同的重要原因之一。
In this study, Pumai 9 and Ningmai 14 were used to investigate the effects of low nitrogen stress on the membrane lipid composition of middle and lower leaves of wheat (Triticum aestivum) at seedling stage. The results showed that the decrease of biomass of ’Pumai 9’ was significantly lower than that of ’Ningmai 14’ after low nitrogen treatment. The chlorophyll content, maximum photochemical efficiency of PSII and photosynthetic rate Are significantly higher than ’Ningmai 14’. Under low nitrogen stress, the content of monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and total lipid in leaves of two wheat cultivars decreased significantly, but ’Puma 9’ DGDG content, DGDG / MGDG ratio, and the double bond index (DBI) of unsaturated fatty acids of MGDG, phospholipid (PL) and total lipids in leaves were significantly higher than those of ’Ningmai 14’. Thus, the different changes of membrane lipid components and their contents in wheat leaves after low nitrogen stress may be one of the important reasons that the two wheat cultivars respond differently to low nitrogen stress.