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本文应用元素分析仪及稳定同位素质谱仪联用技术对黄渤海刺参的碳、氮稳定同位素组成进行分析,同时,通过潜在食物源对刺参生长的贡献率进行计算,研究两海域刺参稳定同位素组成特征及其原因。刺参碳、氮稳定同位素测定结果显示,渤海刺参δ~(13)C值、δ~(15)N值的分布范围分别为-15.660‰~-12.027‰和7.463‰~11.238‰;黄海刺参δ~(13)C值、δ~(15)N值的分布范围分别为-16.062‰~-12.527‰和3.966‰~7.243‰。两海域刺参δ~(15)N值分布范围存在明显差异,表明δ~(15)N值能够有效区分黄渤海不同刺参样品。食源贡献率计算结果显示,渤海刺参食源相对贡献率依次为浮游生物、SOM、POM、海带和孔石莼;黄海刺参食源相对贡献率依次为孔石莼、海带、POM、SOM和浮游生物。食源相对贡献率的不同为两海域刺参δ~(15)N值的差异提供了依据。相关结论能为稳定同位素技术应用于刺参的产地溯源研究提供理论依据。
In this paper, the carbon and nitrogen stable isotopic composition of the sea cucumber in the Bohai Sea and Bohai Sea was analyzed by the combination of elemental analysis and stable isotope mass spectrometry. At the same time, the contribution of potential food sources to the growth of sea cucumber was calculated. Isotope composition and its causes. The results of the carbon and nitrogen stable isotope measurements showed that the distribution ranges of δ ~ (13) C and δ ~ (15) N values were -15.660 ‰ ~ -12.027 ‰ and 7.463 ‰ ~ 11.238 ‰, respectively. The δ ~ (15) N values ranged from -16.062 ‰ to -12.527 ‰ and from 3.966 ‰ to 7.243 ‰, respectively. The distribution of δ ~ (15) N value in two sea areas is obviously different, which shows that δ ~ (15) N value can effectively distinguish different sea cucumber samples from the Huanghai Bohai Sea. The results showed that the relative contribution rates of sea cucumber were Phytoplankton, SOM, POM, kelp and Castanopsis kawakamii, respectively. The relative contribution rates of A. japonicus to A. angustifolia were Aegilops kamii, kelp, POM, SOM And plankton. The difference of the relative contribution rate of food sources provided the basis for the difference of δ ~ (15) N values of two sea cucumbers. The relevant conclusions can provide a theoretical basis for the application of stable isotope techniques in origin tracing of sea cucumber.