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利用ＳＤＳ—ＰＡＧＥ方法对我国１８ 个苜蓿地方品种和北美９ 个苜蓿基本种质来源的代表品种各２０ 粒单粒种子贮藏蛋白及１４ 份豆科牧草种（品种）种子贮藏蛋白进行了分析，根据电泳分析结果计算品种间的欧氏遗传距离，并进行聚类分析。１４ 份豆科牧草种（品种）种子贮藏蛋白的电泳分析结果表明，种内种子贮藏蛋白带谱差异较小，同属不同种间的差异明显，而属间的差异明显大于种间。在聚类分析中，以３．６１ 的遗传距离划分时，１４ 份材料被分为４ 组，分属于４ 个属，这与分类上是一致的。我国１８ 个苜蓿地方品种间的平均遗传距离为０．５７，以０．５ 的遗传距离进行划分时，１８ 个品种可分为７ 组，品种间亲缘关系的远近与品种的地理分布有一定的相关性。北美９ 个苜蓿品种间的平均遗传距离为０．８９，聚类结果表明，杂花苜蓿（Ｍｅｄｉｃａｇｏｓａｔｉｖａ ｓｓｐ． ｖａｒｉａ）与紫花苜蓿（Ｍｅｄｉｃａｇｏ ｓａｔｉｖａ ｓｓｐ． ｓａｔｉｖａ）之间的遗传差异相对较小，而与黄花苜蓿（Ｍｅｄｉｃａｇｏ ｓａｔｉｖａ ｓｓｐ． ｆａｌｃａｔａ）间的遗传差异相对较大。与北美９ 个苜蓿品种相比，我国苜蓿地方品种间的遗传差异较小，这与我国苜蓿地方品种的地理分布相对较窄，品种间基
The SDS-PAGE method was used to analyze the storage proteins of 20 single grain seeds and 14 leguminous species (varieties) of 18 alfalfa cultivars in China and 9 representative cultivars of common alfalfa in North America. According to The results of electrophoresis analysis of the Euclidean distance between varieties, and cluster analysis. Electrophoretic analysis of the storage proteins in 14 leguminous species (varieties) showed that there was little difference in the bands of seed storage protein in the species, with significant differences among different species, while the interspecific differences were significantly greater than those between species. In the cluster analysis, when divided by the genetic distance of 3.61, 14 materials were divided into 4 groups, belonging to 4 genera, which is consistent with the classification. The average genetic distance between 18 local alfalfa cultivars in China was 0.57. When the genetic distance was 0.5, 18 cultivars could be divided into 7 groups. The genetic distance between cultivars and the geographical distribution of cultivars were also certain Correlation. The average genetic distance among 9 alfalfa cultivars in North America was 0.89. The clustering results showed that the genetic differences between Medicago sativa ssp. Varia and Medicago sativa ssp. Sativa were relatively small, Genetic differences between Medicago sativa ssp. Falcata are relatively large. Compared with the 9 alfalfa varieties in North America, the genetic differences among local alfalfa cultivars in China are relatively small, which is relatively narrow with the geographical distribution of alfalfa local cultivars in China.