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以依兰香(Canangaodorata)的花瓣及雄蕊群为外植体,在1/2MS+2,4-D1-2+Kt0.2的培养基上分别诱导出白色和红色的两种愈伤组织,建立了依兰香细胞的大量培养(包括固体培养,悬浮培养及发酵罐培养)体系和单细胞克隆,通过化学方法进行高产油细胞系的初步筛选,从培养细胞中分离出依兰油,对油成分作了详细的分析。
Two kinds of white and red callus were induced on the medium of 1 / 2MS + 2,4-D1-2 + Kt0.2 by using petal and stamen of Canangaodorata as explants, Aromatic culture of cells (including solid culture, suspension culture and fermenter culture) system and single cell cloning, chemical methods for high-yielding oil cell line screening, isolated from the cultured cells ylang ylang, made on the oil composition detailed analysis.