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目的建立了一种高效液相色谱同时分离和测定大气颗粒物PM2.5中16种优控多环芳烃的方法。方法采用二氯甲烷超声提取,浓缩后经乙腈定容,以乙腈和水作为流动相,反相色谱法梯度淋洗分离后,由紫外检测器串联荧光检测器检测。结果 16种PAHs的分离效果较好,线性关系良好(r>0.999),平均回收率为77.5%~104.0%,相对标准偏差为1.1%~6.8%,LOD为0.1μg/L~2.3μg/L,LOQ为0.3μg/L~7.5μg/L,均符合方法学的要求。在采样点采集PM2.5样品,并进行定量分析。通过使用16种PAHs毒性当量因子,计算了各自的毒性当量。结论该方法方便、可靠、灵敏度高,可满足PM2.5中多环芳烃的日常检测要求。
OBJECTIVE To establish a method for the simultaneous separation and determination of 16 optimal PAHs in atmospheric PM2.5 by high performance liquid chromatography. Methods The samples were extracted with methylene chloride by ultrasonic and concentrated by acetonitrile. The mobile phase consisted of acetonitrile and water. The mobile phase was separated by gradient elution with reversed-phase chromatography and detected by ultraviolet detector serial fluorescence detector. Results The 16 PAHs showed good separation and good linearity (r> 0.999) with the average recoveries of 77.5% -104.0%, relative standard deviations of 1.1% -6.8% and LODs of 0.1μg / L ~ 2.3μg / L , LOQ 0.3μg / L ~ 7.5μg / L, are in line with methodological requirements. PM2.5 samples were collected at sampling points and quantified. The respective toxic equivalents were calculated by using 16 PAHs Toxicity Equivalents. Conclusion The method is convenient, reliable and sensitive. It can meet the routine testing requirements of PAHs in PM2.5.