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目的 :研究Yaa基因与骨髓B细胞系细胞的分化发育是否有相互关系。方法 :用Whitlock Witte法从骨髓细胞中培养纯化出B细胞系细胞 ,继而检测B细胞系细胞对脂多糖 (lipopolysaccharide,LPS)刺激后的表型、增殖反应和抗DNA抗体的产生以及对地塞米松诱导后的凋亡情况。结果 :用Whitlock Witte法成功地从骨髓细胞中培养分离出B细胞系细胞。经LPS刺激后 ,雌雄BXSB小鼠B细胞系细胞膜表面CD19抗原和膜表面Ig的分布相似 ,都出现较强的增殖反应和产生IgM类型的抗ssDNA和dsDNA抗体 ,但是对地塞米松诱导的凋亡作用不敏感。结论 :Yaa基因对BXSB小鼠骨髓中B细胞系细胞的分化发育无影响 ,提示Yaa基因仅在外周免疫器官的成熟B细胞中表达活性 ;并且BXSB小鼠骨髓中的自身反应性B细胞系细胞未被删除 ,可能是产生自身抗体的内在原因之一。
Objective: To investigate whether there is a correlation between Yaa gene and the differentiation and development of bone marrow B cell line. METHODS: B cell line cells were purified from the bone marrow cells by Whitlock Witte method. Then the phenotypes, proliferative responses and anti-DNA antibody production of the B cell lines stimulated by lipopolysaccharide (LPS) Induced apoptosis after mitomycin treatment. Results: B cell line cells were successfully isolated from bone marrow cells by the Whitlock Witte method. After LPS stimulation, the distribution of CD19 antigen and membrane surface Ig on the cell membrane of B cell line BXSB in both male and female BXSB mice were similar. Both of them showed strong proliferative responses and produced anti-ssDNA and dsDNA antibodies of IgM type. However, dexamethasone induced apoptosis Death effect is not sensitive. Conclusion: The Yaa gene has no effect on the differentiation and development of B cell lines in bone marrow of BXSB mice, suggesting that Yaa gene expresses its activity only in mature B cells of peripheral immune organs; and the autoreactive B cell line cells in BXSB mouse bone marrow Not deleted, may be one of the inherent causes of autoantibodies.