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目的为了较全面的了解陈化烟叶的细菌多样性,进一步挖掘其中的有益微生物。方法以种植于玉溪元江县的红大烤烟品种为材料,利用16SrDNA克隆测序技术,系统研究了云南玉溪红塔区和元江县两仓库中不同自然陈化时期烟叶表面可培养微生物的种群结构。结果陈化初期,烟叶表面细菌数量较少,元江县低水分烟叶陈化30d时细菌数量达到高峰;红塔区高水分烟叶陈化4.5个月时细菌数量达最大;经过16SrDNA鉴定,烤烟叶面细菌包括芽孢杆菌属(Bacillus sp.)、肠杆菌属(Enterobacter sp.)、类芽孢杆菌属(Paenibacillus sp.)和泛菌属(Pantoeasp.)等8个细菌属。结论不同时期、不同地点陈化烟叶表面细菌的优势种群不尽相同,而芽孢杆菌属始终为优势种群。另外,研究中分离到的大量可培养细菌,为进一步筛选烟叶表面有益细菌提供了重要线索。
Objective To understand more about the bacterial diversity of aged tobacco and to find out the beneficial microorganisms. Methods The flue-cured tobacco varieties planted in Yuanjiang County of Yuxi were used as materials. The 16S rDNA clone sequencing technique was used to systematically study the population structure of culturable microorganisms on the surface of tobacco in different natural aging periods in Yuxi Hongta District and Yuanjiang County, Yunnan Province. Results In the early stage of aging, the number of bacteria on the surface of tobacco leaves was relatively low. The number of bacteria reached the peak at 30 days after the low-moisture tobacco leaf of Yuanjiang County was aged. The bacteria number reached the maximum after 4.5 months of aging tobacco leaves in Hongta District. After 16S rDNA identification, Including 8 bacterial genera, such as Bacillus sp., Enterobacter sp., Paenibacillus sp. And Pantoeasp. Conclusion The dominant bacteria on the surface of aged tobacco at different periods and locations are different, while the genus Bacillus is always the dominant species. In addition, the large number of culturable bacteria isolated from the study provided important clues for further screening of useful bacteria on the surface of tobacco leaves.