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目的:建立不同产地野生和市售山楂叶的UPLC指纹图谱。方法:采用UPLC法,色谱柱为ACQUITY UPLC BEH C_(18)柱(100 mm×2.1 mm,1.7μm),以乙腈-0.1%甲酸溶液梯度洗脱,柱温30℃,流速0.3 mL/min,检测波长355 nm,通过采用相似度分析、主成分分析(PCA)以及正交偏最小方差判别分析(OPLS-DA)等化学计量分析方法来评估药材的质量。结果:建立了20批不同来源山楂叶的专属性指纹图谱,确定了15个共有峰;不同来源样品之间差异较大,通过OPLS-DA可将野生和市售山楂叶显著分成两类;经过对照品对照可知,酚酸类是其主要的差异标记物,可作为其质量控制指标。结论:该方法的验证结果表明所建立的方法具有较好的重复性,简便可靠,结合模式识别方法可用于山楂叶药材野生与市售的鉴别,发现绿原酸是引起野生与市售山楂叶差异的主要成分,该方法可为山楂叶的质量评价提供新的科学依据。
OBJECTIVE: To establish UPLC fingerprinting of wild and commercially available hawthorn leaves from different origins. Methods: The column was ACQUITY UPLC BEH C 18 column (100 mm × 2.1 mm, 1.7 μm) with gradient elution with acetonitrile-0.1% formic acid. The column temperature was 30 ℃ and the flow rate was 0.3 mL / min. The detection wavelength was set at 355 nm. The quality of medicinal materials was evaluated by stoichiometric methods such as similarity analysis, principal component analysis (PCA) and orthogonal partial least squares analysis of variance (OPLS-DA). Results: 20 fingerprints of hawthorn leaves from different origins were established and 15 common peaks were identified. There were significant differences among the samples from different origins. Wild and commercially available hawthorn leaves could be divided into two categories by OPLS-DA. Control reference shows that phenolic acids is the main difference marker, as its quality control indicators. Conclusion: The validation results of this method show that the established method has good repeatability and is simple and reliable. Combining with pattern recognition method, it can be used for the identification of wild and commercially available hawthorn leaf medicinal herbs. It was found that chlorogenic acid is caused by wild and commercially available hawthorn leaves This method can provide a new scientific basis for the quality evaluation of hawthorn leaves.