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目的构建白介素-6报告基因(IL-6reporter)载体并研究活动期系统性红斑狼疮(Systemic Lupus Erythema-tosus,SLE)患者血清对IL-6表达的影响。方法利用标准的分子克隆技术方法将IL-6的启动子区(-590～-1)重组到pGL3-basic载体中得到IL-6reporter,然后转染细胞并研究活动期系统性红斑狼疮患者血清对报告基因活性的影响。结果IL-6reporter的活性达到空载体的5倍左右,而活动期系统性红斑狼疮患者血清能使IL-6reporter的活性进一步升高(1.43±0.53)倍,而正常对照的血清对IL-6reporter无明显的增强作用。结论在活动期系统性红斑狼疮患者血清中存在有促进细胞表达IL-6的因素存在。
Objective To construct interleukin-6 reporter gene (IL-6reporter) vector and study the effect of sera of patients with active systemic lupus erythematosus (SLE) on IL-6 expression. Methods IL-6reporter was obtained by recombining the promoter region of IL-6 (-590 ~ -1) into pGL3-basic vector using standard molecular cloning techniques. Then the cells were transfected and the serum levels of active IL-6 in patients with active systemic lupus erythematosus Effects of reporter gene activity. Results The activity of IL-6reporter was about 5 times that of the empty vector, while the serum of patients with active systemic lupus erythematosus could further increase the activity of IL-6reporter (1.43 ± 0.53) times, while the serum of normal control had no effect on IL-6reporter Obvious enhancement. Conclusion There are some factors that promote the expression of IL-6 in sera of patients with active systemic lupus erythematosus.