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探讨CD25和Foxp3分子表达水平与自发性流产小鼠模型流产发病机制的关系。采用磁珠亲和细胞分选术分离小鼠子宫NK细胞,并采用流式细胞术检测NK细胞表达CD25和Foxp3分子的情况,比较自发性流产小鼠CBA/J×DBA/2J和正常对照CBA/J×BALB/c小鼠流产率和上述分子表达水平的差异。在孕早期给CBA/J×DBA/2J雌鼠过继输注CD49~+CD25~+Foxp3~+NK细胞,观察其胚胎丢失率的变化。结果显示,CBA/J×DBA/2J小鼠孕12.5 d胚胎吸收率显著高于CBA/J×BALB/c对照组(分别为23.4%和5.5%,P<0.01),而CBA/J×DBA/2J小鼠孕12.5 d子宫NK细胞CD25和Foxp3阳性率均显著低于CBA/J×BALB/c小鼠(CD25阳性率分别是4.8%和10.5%,Foxp3阳性率分别是0.3%和7.4%,均为P<0.01)。进一步检测发现,这些细胞不表达CD3和CD4分子。此外,输注CD49~+CD25~+Foxp3~+NK细胞可显著降低CBA/J×DBA/2J小鼠孕12.5 d胚胎吸收率(治疗组与对照组分别为8.0%和24.7%,P<0.01)。这些结果提示,CBA/J×DBA/2J小鼠自发性胚胎吸收率增高可能与子宫NK细胞缺乏CD25和Foxp3分子的足量表达有关。
To investigate the relationship between the expression of CD25 and Foxp3 and the pathogenesis of spontaneous abortion mouse model of abortion. The method of magnetic beads affinity cell sorting was used to isolate mouse uterine NK cells. Flow cytometry was used to detect the expression of CD25 and Foxp3 in NK cells. Compared spontaneous abortion mouse CBA / J × DBA / 2J with normal control CBA / J × BALB / c mouse abortion rate and the above molecular expression differences. CBA / J × DBA / 2J female mice were transfused with CD49 ~ + CD25 ~ + Foxp3 ~ + NK cells in the first trimester of pregnancy, and their embryo loss rate was observed. The results showed that the embryo uptake rate of CBA / J × DBA / 2J mice at 12.5 d of pregnancy was significantly higher than that of CBA / J × BALB / c control groups (23.4% and 5.5%, respectively, P <0.01) The positive rates of CD25 and Foxp3 in uterus NK cells in / 2J mice were significantly lower than those in CBA / J × BALB / c mice at 12.5 d of pregnancy (CD25 positive rate was 4.8% and 10.5%, Foxp3 positive rates were 0.3% and 7.4% , All P <0.01). Further testing revealed that these cells did not express CD3 and CD4 molecules. In addition, transfusion of CD49 ~ + CD25 ~ + Foxp3 ~ + NK cells could significantly reduce embryonic 12.5-day embryo uptake in CBA / J × DBA / 2J mice (8.0% vs. 24.7%, P <0.01 ). These results suggest that increased spontaneous embryo uptake in CBA / J × DBA / 2J mice may be related to the lack of adequate expression of CD25 and Foxp3 molecules in uterine NK cells.