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用荧光光谱法、紫外-可见分光光度法在生理条件下(pH=7.41)研究了β-环糊精(β-CD)、6-去氧-(N-胺乙基)环糊精(CR-β-CDen)分别与刚果红(CR)的包合作用;研究了CR,(CR-β-CD)和(CR-β-CDen)超分子体系与小牛胸腺DNA的相互作用。紫外滴定实验结果表明:(1)β-环糊精和6-去氧-(N-胺乙基)环糊精都与客体分子刚果红发生超分子作用;(2)CR,CR-β-CD,CR-β-CDen与小牛胸腺DNA作用的结合常数Kb值分别为4.1×104L/mol,1.36×105L/mol,2.33×105L/mol,表明超分子体系CR-β-CDen与DNA的相互作用最强。荧光实验结果表明三种化合物CR,CR-β-CD,CR-β-CDen的荧光强度随着CT-DNA的增加而增大,其中环糊精衍生物CR-β-CDen荧光增强效果是最好的。
The effects of β-cyclodextrin (β-CD), 6-deoxy- (N-aminoethyl) cyclodextrin (CR) (CR-β-CD) and (CR-β-CDen) and Congo red (CR) respectively. The interaction between CR, (CR-β-CD) and (CR-β-CDen) supramolecular system and calf thymus DNA was studied. The results of UV titration showed that: (1) both β-cyclodextrin and 6-deoxy- (N-aminoethyl) cyclodextrin had supramolecular interactions with guest congo red; (2) CR, The binding constant values of CD, CR-β-CDen and calf thymus DNA were 4.1 × 104 L / mol, 1.36 × 105 L / mol and 2.33 × 105 L / mol, respectively. The strongest interaction. Fluorescence results showed that the fluorescence intensities of CR, CR-β-CD and CR-β-CDen increased with the increase of CT-DNA, and the CR-β-CDen fluorescence enhancement effect of cyclodextrin derivatives was the most Ok.