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用抗人细小病毒Ｂ１９的ＭｃＡｂ建立ＥＬＩＳＡ－抗γ捕捉法，对正常新生儿（３５例）脐血标本及１月龄～１３岁小儿（１３９例）血清标本计１７４份，进行了Ｂ１９病毒特异性ＩｇＧ检测，结果阳性率分别为２２．９％（８／３５）和５．８％（８／１３９）．总阳性率为９．０％，并且大多数阳性标本的特异性ＩｇＧ抗体水平较低，最高滴度约为１：４００。结果显示：本文建立的检测方法有高度特异性；我国小儿中有Ｂ１９病毒自然感染，并有较高数量的易感人群，应进一步深入研究Ｂ１９病毒对我国小儿的危害及其防护措施。
Using anti-human parvovirus B19 McAb to establish ELISA-anti-gamma capture method, 174 samples of normal neonatal (35 cases) cord blood samples and 139 cases of children aged from 1 month to 13 years old were assayed for B19 virus specificity Sex IgG, the positive rates were 22.9% (8/35) and 5.8% (8/139) respectively. The total positive rate was 9.0%, and the majority of positive specimens had lower specific IgG antibodies with a titer of about 1: 400. The results showed that the detection method established in this paper was highly specific. In our country, there was a natural infection of B19 virus in children and a higher number of susceptible populations. The harm of B19 virus to our children and its protective measures should be further studied.