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在无血清８４１培养基中分别添加胸腺嘧啶核苷（１６×１０－５ｍｏｌ／Ｌ）、鸟氨酸（１０ｍｇ／Ｌ）、强的松龙（１０－５ｍｏｌ／Ｌ）和胰蛋白胨（１０ｇ／Ｌ），可促进日本血吸虫产卵。经１５ｄ的观察证实在８４１培养基中添加上述成份组成的８９１培养基比８４１培养基产卵量高、畸卵率低和产卵高峰持续时间长。成虫最初２４ｈ内产出的虫卵体外培养至第１５ｄ，ＲＰＭＩ１６４０培养基、无血清８４１培养基、８４１培养基和８９１培养基上成熟期虫卵的百分比依次为１１６±４７，１８７±５６，２８４±８３和３０１±７８。
Thymidine (16 × 10-5mol / L), ornithine (10mg / L), prednisolone (10-5mol / L) and tryptone 0g / L), can promote Schistosoma japonicum spawning. After 15 days of observation, it was confirmed that the 891 medium containing the above-mentioned components in 841 medium had higher ovulation amount, lower ovulation rate and longer spawning peak than 841 medium. The eggs accumulated in the first 24 h of adult worms were cultured in vitro to the percentage of mature eggs on the 15th day in RPMI1640 medium, serum-free 841 medium, 841 medium and 891 medium, 116 ± 47,18 7 ± 56, 284 ± 83 and 301 ± 78.