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目的:探讨黄连合用肉桂多次灌胃给予大鼠对小檗碱体内分布的影响。方法:将大鼠随机分为3组,一组作为空白,一组灌服黄连药材粉末,另一组灌服黄连与肉桂按6∶1混合的药材粉末,连续灌胃7次。采用HPLC测定给药后大鼠心、肝、脾、肺、肾等脏器中小檗碱的浓度。采用SAS统计软件进行t检验,比较黄连配伍肉桂前后各脏器中小檗碱的变化情况。结果:大鼠多次灌胃黄连后,小檗碱组织浓度升高,其在大鼠体内呈广泛分布,但主要集中在肝组织中;黄连配伍肉桂后各脏器中小檗碱浓度发生明显的改变(P<0.01),心、肝、肾中小檗碱浓度较之配伍前明显增加,而脾、肺中小檗碱浓度较配伍前明显减少。结论:黄连配伍肉桂后多次灌胃可使小檗碱在大鼠体内各脏器的分布发生明显的改变。
Objective: To investigate the effect of berberine on the distribution of berberine in rats given multiple times by intragastric administration of Coptidis Rhizoma Coptidis. Methods: The rats were randomly divided into three groups. One group served as a blank group. One group was fed with Coptis powder and the other group was treated with herbal powder mixed with Coptis chinensis and Cinnamon at 6: 1 for 7 times. The concentrations of berberine in heart, liver, spleen, lung, kidney and other organs were determined by HPLC after administration. Using SAS statistical software for t test, compared with Coptis before and after cinnamon in various organs of berberine changes. Results: After repeated intragastric administration of berberine, the concentration of berberine increased, which was widely distributed in rats, but mainly concentrated in the liver tissue. After berberine combined with berberine, the concentration of berberine significantly (P <0.01). The concentrations of berberine in heart, liver and kidney were significantly increased compared with that before compatibility, while the concentration of berberine in spleen and lung was significantly decreased compared with that before compatibility. Conclusion: Coptis chinensis after multiple intragastric administration of berberine can significantly change the distribution of each organ in rats.