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目的 筛选胃癌 19p部分微卫星多态位点的杂合性缺失 (loss of heterozygosite,L OH)频率 ,以初步确定 19p上与胃癌相关基因连锁最密切的微卫星多态位点。方法 采用聚合酶链反应 -单链长度多态(polymerase chain reaction- single strand length polymorphism,PCR- SSL P) -银染法选取 19p上 9对微卫星多态标记 (D19S42 4,D19S2 16 ,D19S40 6 ,D19S413,D19S2 2 1,D19S2 2 6 ,D19S411,D19S883,D19S886 ) ,对 43例原发性胃癌的杂合性缺失情况进行了分析。结果 43例中 2 2例至少在 1个位点发生 L OH,总缺失率为48.88%,这 9个位点的 L OH频率分别为 2 9.6 3%,11.5 3%,33.33%,8.5 7%,13.15 %,8.0 0 %,6 .45 %,6 .89%,10 .71%,在 D19S886也同时出现微卫星不稳定性 (microsatellite instability,MSI) 17.85 %。结论 提示 19p上的 L OH缺失频发区域可能涉及与人类原发性胃癌发生发展相关基因的存在。
Objective To screen the loss of heterozygosity (L OH) frequencies of 19p partial microsatellite polymorphic loci in order to identify the most closely related microsatellite polymorphic sites on gastric cancer related genes on 19p. Methods Polymerase chain reaction-single strand length polymorphism (PCR-SSL P)-silver staining method was used to select 9 pairs of microsatellite polymorphism markers on 19p (D19S42 4, D19S2 16, D19S40 6 , D19S413, D19S2 21, D19S2 26, D19S411, D19S883, D19S886), analysis of heterozygous loss in 43 cases of primary gastric cancer. Results LOH was found in at least 1 locus in 22 out of 43 patients. The total deletion rate was 48.88%. The L OH frequencies of these 9 loci were 2 9.6 3%, 11.5 3%, 33.33%, and 8.5 7%, respectively. , 13.15 %, 8.0 0 %, 6.45%, 6.99%, 10.71%, and the microsatellite instability (MSI) was 17.85 % at D19S886. Conclusions The frequent loss of LOH on 19p may involve the presence of genes associated with the development of human primary gastric cancer.