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目的检测28株儿童临床分离的鲍曼不动杆菌(AB菌),研究其产生blaADC型的头孢菌素酶的基因型。方法收集2006年分离自儿科临床的28株AB菌,采用VITEK-32全自动微生物鉴定仪GNI和GNS卡进行细菌鉴定和药敏试验。blaADC基因检测用聚合酶链反应(PCR)方法及序列分析确定其基因型。结果检测的28株AB菌有3株呈多重耐药性,阳性率10.71%,bla ADC检出17株,阳性率60.71%,28株菌对头孢西丁均耐药,bla ADC阳性菌株中对氨苄西林/舒巴坦均敏感,哌拉西林/他唑巴坦只有1株耐药,而对氨苄西林/舒巴坦和哌拉西林/他唑巴坦耐药的菌株中并未分离出bla ADC。2号株的bla ADC序列与GenBank中的gi|7258342|emb|CAB77444.1|相比在第4位、第242位、第342位、第376位氨基酸发生了改变。结论儿童临床分离的AB菌60%以上携带bla ADC,在所携带的bla ADC中随机抽取1株进行了核苷酸序列分析,发现与目前在GenBank中登录的国内外型别均不同,为新亚型。
Objective To detect clinically isolated Acinetobacter baumannii (AB) from 28 children and study the genotypes of cephalosporins producing blaADC. Methods The 28 strains of bacteria isolated from pediatrics in 2006 were collected and the bacteria were identified and susceptible to antibiotics by using VITEK-32 automatic microbial identification device GNI and GNS card. The blaADC gene was detected by polymerase chain reaction (PCR) and sequence analysis to determine its genotype. Results The results showed that 3 strains of 28 strains were multi-drug resistant, the positive rate was 10.71%, the number of bla ADC was 17, the positive rate was 60.71%. 28 strains were resistant to cefoxitin, Ampicillin / sulbactam were sensitive, and only 1 strain was resistant to piperacillin / tazobactam, whereas none of the strains resistant to ampicillin / sulbactam and piperacillin / tazobactam was isolated ADC. The bla ADC sequence of strain No. 2 was changed at positions 4, 242, 342 and 376 compared to gi | 7258342 | emb | CAB77444.1 in GenBank. CONCLUSIONS: Over 60% of clinically isolated AB bacteria carry bla ADC, and one randomly selected bla ADC is carried out for nucleotide sequence analysis. The results showed that they were different from those currently registered in GenBank and were new Subtype.