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【目的】增强真菌预处理的效率和降低热水预处理对反应条件的要求。【方法】综合利用白腐菌和热水预处理毛白杨,分析此方法对毛白杨化学组分和酶水解效果的影响。【结果】白腐菌Lenzites betulinus C5617协同热水处理,损失率最高达70.70%。纤维素在2个预处理阶段都有损失,其中L.betulinus C5617达到29.62%。木质素的降解主要集中在白腐菌预处理阶段,其中L.betulinus C5617降解的酸不溶木素较多,达到了16.98%。综合预处理显著改善了毛白杨的酶水解效果。与只经热水预处理的样品相比较,L.betulinus C5617和P.sanguineus D9497协同热水处理分别引起还原糖得率上升了20.60%和12.23%。【结论】综合预处理降低了热水解对反应条件的要求,节约了预处理成本。
【Objective】 The objective of this study was to enhance the efficiency of fungal pretreatment and to reduce the reaction conditions for hot water pretreatment. 【Method】 The white poplar was pretreated with white-rot fungi and hot water, and the effects of this method on chemical constituents and enzymatic hydrolysis of Populus tomentosa were analyzed. 【Result】 The white rot fungus Lenzites betulinus C5617 synergistic with hot water treatment, the loss rate was up to 70.70%. Cellulose lost at both pretreatment stages, with L.betulinus C5617 reaching 29.62%. The degradation of lignin mainly concentrated in the stage of pretreatment of white-rot fungi, in which L.betulinus C5617 degraded more acid-insoluble lignin, reaching 16.98%. Comprehensive pretreatment significantly improved the enzymatic hydrolysis of Populus tomentosa. Compared with the samples pretreated with hot water only, synergistic hot water treatment of L.betulinus C5617 and P. sanguineus D9497 respectively increased the yield of reducing sugars by 20.60% and 12.23%, respectively. 【Conclusion】 The comprehensive pretreatment reduced the requirements for the reaction conditions by thermal hydrolysis and saved the pretreatment cost.