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目的:建立同时测定鸡肉参中芦丁、槲皮苷、槲皮素含量的HPLC-DAD方法。方法:采用Diamonsil-C18色谱柱(150 mm×4.6 mm,5.0μm),柱温30℃,以甲醇(A)-0.5%磷酸水溶液(B)为流动相,进行梯度洗脱(0～5 min,48%～52%A;6～10 min,70%～30%A),检测波长254 nm,流速1.0 mL.min-1。结果:3种黄酮类成分在10 min内达到良好分离,芦丁、槲皮苷、槲皮素的线性范围分别为0.3～1.0 mg(r=0.999 8),0.030～0.10 mg(r=0.999 8),0.010～0.033 mg(r=0.999 5);平均加样回收率分别为100.95%(RSD 0.98%),99.51%(RSD 1.54%),102.84%(RSD 3.53%)。结论:该方法简便,快速,精确,可同时测定鸡肉参中芦丁、槲皮苷、槲皮素的含量,具有较好的重复性和稳定性,可作为鸡肉参药材的质量控制方法。
Objective: To establish a HPLC-DAD method for simultaneous determination of rutin, quercitrin and quercetin in chicken ginseng. METHODS: Diamonsil-C18 column (150 mm × 4.6 mm, 5.0 μm) was used. The column temperature was 30 ℃ and the mobile phase was methanol (A) -0.5% phosphoric acid (B) , 48% -52% A, 6-10 min, 70% -30% A). The detection wavelength was 254 nm and the flow rate was 1.0 mL.min-1. RESULTS: The three flavonoids were well separated within 10 min. The linear range of rutin, quercitrin and quercetin were 0.3 ~ 1.0 mg (r = 0.999 8) and 0.030 ~ 0.10 mg (r = 0.999 8 ) And 0.010-0.033 mg (r = 0.999 5) respectively. The average recoveries were 100.95% (RSD 0.98%), 99.51% (RSD 1.54%) and 102.84% (RSD 3.53%), respectively. Conclusion: The method is simple, rapid and accurate and can be used for the simultaneous determination of rutin, quercitrin and quercetin in chicken ginseng. It has good repeatability and stability and can be used as a quality control method for chicken ginseng.