论文部分内容阅读
目的 探讨反义MDR核酸技术联合化疗对耐药肿瘤细胞的生长抑制作用。方法 选用人耐药肿瘤细胞KBv2 0 0 ,插入4 17bp的反义MDRmRNA片段 ,构建反义MDR载体 ,并转染KBv2 0 0 ,形成稳定表达的反义MDRmRNA的KBv2 0 0细胞株 ,加入化疗药物VCR ,HCPT并与正义MDRmRNAKBv2 0 0及KBv2 0 0对比 ,经MTT ,流式细胞仪PI染色法 ,荧光显微镜HT染色法及AnnexinV检测其抑制率及凋亡率。结果 反义MDRmRNA应用VCR ,HCPT后与正义组及对照组相比 ,抑制率和凋亡率均有明显升高。结论 反义MDR寡核苷酸联合化疗药物能有效抑制耐药肿瘤细胞生长 ,体外实验中证实了基因治疗可逆转肿瘤细胞对VCR ,HCPT等化疗药物的多药耐药性。
Objective To investigate the antitumor effect of antisense MDR nucleic acid combined with chemotherapy on the growth of drug-resistant tumor cells. METHODS: Human antitumor MDR mRNA fragment was inserted into human multidrug resistance cell line KBv2 0 0, antisense MDR mRNA fragment was inserted into antisense MDR vector and transfected into KBv2 0 0 cells to form stably expressed antisense MDR mRNA of KBv2 0 0 cells. Chemotherapy drugs VCR and HCPT were compared with those of MDR mRNA KBv20 0 and KBv2 0 0. The inhibitory rate and apoptosis rate were detected by MTT assay, flow cytometry PI staining and fluorescence microscopy with Annexin V staining. Results After antisense MDR mRNA was applied to VCR and HCPT, the inhibitory rate and apoptosis rate of VCR and HCPT were significantly increased compared with those of the normal control group and the control group. Conclusion Antisense MDR oligodeoxynucleotides combined with chemotherapeutic agents can effectively inhibit the growth of drug-resistant tumor cells. In vitro experiments show that gene therapy can reverse the multidrug resistance of tumor cells to chemotherapeutic drugs such as VCR and HCPT.