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采用HPLC法,对医院制剂归芍合剂中主药当归的阿魏酸的含量进行了测定。以甲醇-四氢呋喃-水-醋酸(20:10:70:0.5→30:10:70:0.5)梯度洗脱,梯度变化速率为0.285%/min,其它成分对测定无干扰。样品回收率为98.5%。
The HPLC method was used to determine the content of ferulic acid, the main drug in the hospital formulation. Elution with a gradient of methanol-tetrahydrofuran-water-acetic acid (20:10:70:0.5→30:10:70:0.5) with a gradient rate of 0.285%/min. No interference with other components . The sample recovery was 98.5%.