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一、实验共用成年雄性大白鼠49只,体重为140-210克。其中9只不加任T可处理作为对照。其余40只分别经臀部肌肉注射可的松或氢化可的松25毫克/每日一次。注射分一次、三次、六次以及注六次后恢复4,5和9日后杀死取材。二、随注射次数的增加和实验时间的延长大鼠脾脏逐渐萎缩。到注六次或注六次后恢复4和5日时可缩小到正常的1/3以上。其内部结构也相应地引起了十分明显地变化。红白髓中淋巴细胞迅速减少,到注六次恢复4和5日时脾小体几乎完全消失,红白髓难于辨认。此时整个脾脏几乎被红髓占据。注射六次后恢复9日时,虽有些恢复,但仍未达正常水平。三、实验动物脾脏淋巴细胞中RNA合量减少,尤其大中淋巴细胞较为明显。固缩和破碎的淋巴细胞核普遍存在,尤其脾小体处为多,有些已被亘噬细胞吞噬。而中性粒细胞的数量增加,井多量的堆积于脾小体周围。旦噬细胞及其吞噬的含铁血黄素显著增多。 蛋白质、蛋白质结合一SH和DNA含量未见变化。四、磷酸酶的活性变化,以ALP和ACP较为显著。ALP在注射初期升高,以注射三次者为最明显,随后酶活性一值下降。而ACP活性在实验时期一直上升。前者以中性粒细胞为代表,后者以网状细胞为代表。三磷酸腺普酸酶在注射初期上升,之后则逐渐恢复正常。5一核试酸酶、非特异性磷酸酶和醋酶的活性虽有变化,但不明显。
First, the experiment shared 49 adult male rats, weighing 140-210 grams. Nine of them were served as controls without any T treatment. The remaining 40 were injected intramuscular cortisol or hydrocortisone 25 mg / once daily. Injection once, three times, six times, and after the injection of the restoration of 4,5 and 9 days after the killing of drawing. Second, with the increase in the number of injections and experimental extension of the spleen of rats gradually atrophy. It can be reduced to the normal 1/3 or more when it is resumed for 4 and 5 after six or six times. Its internal structure also caused a very significant change accordingly. Red white pulp lymphocytes rapidly decreased to note six times recovery 4 and 5 spleen body almost completely disappeared, red and white marrow difficult to identify. At this point the spleen is almost occupied by the red pulp. Nine times recovery after injection six times, although some recovery, but still not up to normal levels. Third, the experimental animals, spleen lymphocytes in the amount of reduced RNA, especially in large lymphocytes is more obvious. Systole and broken lymphocyte nuclei are ubiquitous, especially splenomegaly, and some have been phagocytic phagocytes. The number of neutrophils increased, a lot of well accumulation in the spleen around the body. Daphnocytes and phagocytic hemosiderin was significantly increased. Protein, protein binding of SH and DNA content showed no change. Fourth, changes in phosphatase activity to ALP and ACP more significant. ALP increased in the early injection to the injection of the three were the most obvious, followed by a decrease in enzyme activity. The ACP activity has been rising during the experimental period. The former is represented by neutrophils, the latter by reticular cells. Adenosine triphosphate enzyme in the early stages of injection, then gradually returned to normal. 5 nuclear nuclease, non-specific phosphatase and enzyme activity despite the change, but not obvious.