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目的研究妊娠期高血压疾病(hypertensive disorder complicating pregnancy,HDCP)与胰岛素样生长因子-Ⅰ(insulin-like growth factor-Ⅰ,IGF-Ⅰ)、胰岛素样生长因子-Ⅱ(insulin-like growth factor-Ⅱ,IGF-Ⅱ)、胰岛素样生长因子结合蛋白-1(insulin-like growth factor binding protein-1,IGFBP-1)的相关性,为HDCP的病因学研究提供理论依据。方法采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测脐血和羊水中IGF-Ⅰ、IGF-Ⅱ、IGFBP-1水平。正常孕妇16例(对照组),HDCP孕妇48例(研究组),其中妊娠期高血压17例,轻度子痫前期16例,重度子痫前期15例。结果研究组较对照组IGF-Ⅰ、IGF-Ⅱ水平均显著下降(P<0.05)、IGFBP-1水平显著升高(P<0.05);研究组内IGF-Ⅰ和IGF-Ⅱ水平随病情程度加重而降低、IGFBP-1水平随病情程度加重而升高,不同病情程度之间比较差异有统计学意义(P<0.05)。研究组IGF-Ⅰ和IGF-Ⅱ呈正相关,IGF-Ⅰ、IGF-Ⅱ均与IGFBP-1呈负相关(P<0.05)。结论IGF-Ⅰ、IGF-Ⅱ、IGFBP-1与HDCP的发生和病情严重程度密切相关。
Objective To investigate the relationship between HDCP and insulin-like growth factor-Ⅰ (IGF-Ⅰ), insulin-like growth factor-Ⅱ , IGF-Ⅱ) and insulin-like growth factor binding protein-1 (IGFBP-1), provide a theoretical basis for the etiological study of HDCP. Methods The levels of IGF-Ⅰ, IGF-Ⅱ and IGFBP-1 in umbilical cord blood and amniotic fluid were detected by enzyme linked immunosorbent assay (ELISA). 16 normal pregnant women (control group) and 48 HDCP pregnant women (study group), of which 17 cases of gestational hypertension, mild preeclampsia in 16 cases and severe preeclampsia in 15 cases. Results The levels of IGF-Ⅰ and IGF-Ⅱ in the study group were significantly decreased (P <0.05) and IGFBP-1 levels were significantly increased (P <0.05). The levels of IGF-Ⅰ and IGF-Ⅱ in the study group were significantly correlated with the severity of the disease The level of IGFBP-1 increased with the severity of illness, and the difference was statistically significant (P <0.05). There was a positive correlation between IGF-Ⅰ and IGF-Ⅱ in the study group, IGF-Ⅰ and IGF-Ⅱ were negatively correlated with IGFBP-1 (P <0.05). Conclusion The occurrence of IGF-Ⅰ, IGF-Ⅱ, IGFBP-1 and HDCP are closely related to the severity of the disease.