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目的研究麻花头属植物麻花头Serratula chinensis干燥块根的化学成分。方法采用硅胶、Sephadex LH-20、ODS柱色谱和制备HPLC等方法进行分离,并通过理化常数和NMR等各种波谱学方法鉴定化合物结构。结果从广升麻干燥块根95%乙醇提取物的正丁醇部位分离得到了16个化合物,分别鉴定为20-羟基蜕皮甾酮(1)、polypodine B(2)、carthamosterone(3)、20,22-异丙叉基-20-羟基蜕皮甾酮(4)、24-epi-abutasterone(5)、polypodine C(6)、coronatasterone(7)、20-羟基蜕皮甾酮2-O-β-D-葡萄糖苷(8)、20-羟基蜕皮甾酮25-O-β-D-葡萄糖苷(9)、polypodine B 20,22-acetonide(10)、shidasterone(11)、2-O-乙酰基-20-羟基蜕皮甾酮(12)、3-O-乙酰基-20-羟基蜕皮甾酮(13)、20-羟基蜕皮甾酮-20,22-缩丁醛(14)、24-methylene-shidasterone(15)及ajugasterone D(16)。结论化合物2、4、5、7~10、12、15、16为首次从该植物中分离得到,化合物5、7~10、16为首次从麻花头属植物中分离得到。
Objective To study the chemical constituents of dry root of Serratula chinensis in the genus Methods The compounds were isolated by silica gel, Sephadex LH-20, ODS column chromatography and preparative HPLC. Their structures were identified by physicochemical constants and NMR spectroscopy. Results Sixteen compounds were isolated from the n-butanol fraction of 95% ethanol extract of Radix et Rhizoma Drynariae and identified as 20-hydroxyecdysone (1), polypodine B (2), carthamosterone (3) 24-epi-abutasterone (5), polypodine C (6), coronatasterone (7), 20-hydroxyecdysterone 2-O-β-D -glucoside (8), 20-hydroxyecdysone 25-O-β-D-glucoside (9), polypodine B 20,22-acetonide (10), shidasterone (11) (12), 3-O-acetyl-20-hydroxyecdysterone (13), 20-hydroxyecdysterone-20,22-butyraldehyde (14), 24-methylene-shidasterone (15) and ajugasterone D (16). Conclusions Compounds 2, 4, 5, 7 ~ 10, 12, 15 and 16 were isolated from this plant for the first time. Compounds 5, 7 ~ 10 and 16 were isolated from Ephedra for the first time.