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目的:探讨益母草水苏碱(stachydrine,Sta)对去甲肾上腺素(norepinephrine,NE)诱导的新生大鼠心肌细胞肥大肌浆网钙摄取能力的影响。方法:采用不同浓度的益母草水苏碱(10-4、10-5、10-6mol/L)对NE诱导的心肌肥大模型组进行干预,通过大鼠心肌肌浆网的蛋白水平和钙摄取能力来反映益母草水苏碱对NE诱导的新生大鼠心肌细胞肥大的影响。结果:益母草水苏碱能够明显减少心肌细胞蛋白含量,降低Protein/DNA比值;益母草水苏碱可剂量依赖地提高NE诱导的心肌肥大细胞的肌浆网钙摄取功能。结论:益母草水苏碱对NE诱导的心肌肥大细胞的肌浆网钙摄取功能有一定的程度的提高,与剂量有一定的依赖关系,随着益母草水苏碱的浓度的提高,肌浆网的钙摄取速率也明显提高。
Objective: To investigate the effect of stachydrine (Sta) on Ca2 + uptake in sarcoplasmic reticulum of neonatal rat cardiomyocytes induced by norepinephrine (NE). Methods: NE concentration-induced stachyose (10-4,10-5,10-6 mol / L) was used to induce myocardial hypertrophy in rats. The protein level and calcium uptake capacity of sarcoplasmic reticulum To reflect the effects of Leonurus & lt; (R) & gt; stachydrine on cardiomyocyte hypertrophy induced by NE in neonatal rats. Results: Motherwort stachydrine could significantly reduce the protein content of cardiomyocytes and decrease the protein / DNA ratio. Motherwort stachydrine could increase the sarcoplasmic reticulum calcium uptake by cardiac myocytes induced by NE in a dose-dependent manner. Conclusion: Stachyosine of Motherwort on NE-induced myocardial mast cells sarcoplasmic reticulum calcium uptake function to a certain extent, and the dose has a certain dependence, with the concentration of stachydrine stachydrine increased sarcoplasmic reticulum Calcium uptake rate is also significantly increased.