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目前,全国杂交水稻播种面积已占水稻播种总面积的40%,产量则达到水稻总产量的50%以上.随着杂交水稻种植面积不断扩大,原种生产和销售过程中的种子纯度问题显得日趋重要,杂交种子纯度无法单纯从种子形态上分辨,往往需要将种子播种后至成熟期方能辨别,使用纯度低或真实性差的种子会给农业生产造成极大损失.简便、快速、准确和早期地鉴定杂交水稻种子纯度的方法将为稳定杂交水稻的增产效果提供保证.在本研究中,我们使用RAPD(Random amplified polymorphic DNA)技术,针对全国播种面积最大的由雄性不育系珍汕97A为母本,恢复系明恢63为父本制成的杂交水稻汕优63(F1),利用RAPD随机引物扩增筛选出F1与父本相同或与母本相同或与父母本相互补的RAPD特异扩增谱带,并以此特异谱带做为分子标记首次对杂交水稻杂种纯度进行鉴定.
At present, the sown area of hybrid rice in China accounts for 40% of the total sown area of rice and the output reaches more than 50% of the total output of rice.With the increasing acreage of hybrid rice, the problem of seed purity in the production and marketing of original sown Importantly, the purity of hybrid seeds can not be distinguished solely from the seed morphology, and it is often necessary to distinguish the seeds from the sowing date to the mature one. The use of seeds of low purity or poor authenticity can cause great losses to agricultural production. Simple, rapid, accurate and early The method of identifying the purity of hybrid rice seed will provide a guarantee for stabilizing the yield of hybrid rice.In this study, we used random amplified polymorphic DNA (RAPD) Female parent and restorer line Minghui63 as the male parent hybrid rice Shanyou 63 (F1). RAPD-specific random amplified polymorphic DNA (RAPD) was used to screen F1 hybrids with the same or same parental or parental RAPD The bands were amplified and the purity of hybrid rice hybrids was identified for the first time using this specific band as a molecular marker.